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真菌细胞色素P450与多环芳烃浓度及降解率的相互关系

Keywords: 真菌,细胞色素P450,降解,菲浓度,芘浓度

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Abstract:

以菲、芘作为多环芳烃代表污染物,采用室内培养方法,研究了4种真菌细胞色素P450含量与多环芳烃(PAHs)浓度及降解率的相互关系。结果表明,在一定的浓度范围内(菲0~200mg·L-1、芘0~100mg·L-1),菲、芘浓度与真菌P450含量呈明显正相关,菲、芘浓度与真菌P450含量之间表现出明显的剂量-效应关系。在上述浓度内,真菌降解多环芳烃的能力与真菌P450含量之间也表现出明显的剂量-效应关系。在选用的4株真菌中,Zj1(镰刀菌)降解菲和芘的能力最强,Zj3(小克银汉)次之,Zj2(毛霉)和Zj4(青霉)降解能力较弱。

References

[1]  许华夏,李培军,刘宛,等.生物细胞色素 P450的研究进展 [J].农业环境保护,2002, 21( 2): 188-191.
[2]  Launen L, Pinto L, Wiebe c et al.The oxudation of pyrene and benzo (a) pyrene by nonbasidiomycetes fungi[J]. Can J Microbiol,1995,41: 477-488.
[3]  冷欣夫,邱星辉. 生物细胞色素 P450酶系的结构、功能与应用前景 [M].北京:科学出版社, 2001.
[4]  Brattsten L B.Metabolic defenses against plant allelichemicals[A].In: Rosenthal G A and Janzen D.eds.Herbivores; their interactions with secondary plant metabolites[C].2nd edition, Vol.2: evolutionary and ecological process Inc.1992.219-242.
[5]  巩宗强,李培军,等. 污染土壤中多环芳烃的共代谢降解过程 [J]. 生态学杂志,2000,19( 6): 40-45.
[6]  Faber BW, Schonewille AB, van Gorcom RF. JA.Constitutive and inducible hydroxylase activities involved in the degradation of naphtha lene by Cunninghamella elegans[J]. Appl Microbiol Biotechnol, 2001, 55(4): 486-491.
[7]  Ferry F, et al. Review and perspecitive on the use of mixed-function oxygenase enzymes in biological monitoring[J]. Biochem Physiol, 1986,2:233-245.
[8]  宋玉芳,区自清,孙铁珩. 土壤和植物样品中多环芳烃( PAHS)的分析方法研究 [J]. 应用生态学报,1995, 6( 1): 92-96.
[9]  Bradford M M A. Rapid and sensitive method for guantification micro gram quantities of Pooteindye birding[J]. Aral-Biochem, 1979, (72):248-250.
[10]  Omura T, Sato R. The carbon monoxide-binding pigment of liver microsomes,I Evidence for its bemoprotein[J].J Biol Chem,1964,239 (7):2370-2378.
[11]  Hudnik-Plevnik T, Breskvar K. Cytochrome P450 from Rhizopus nigricans. In: Ruckpaul, K et al. Microbial and Plant Cytochrome P450[M]. London: Taylor and Francis, 1991. 149-167.
[12]  Rodriguez-Saiz M, Barredo J L, Moreno M A, Reduced function of a phenylacetate-oxidizing cytochrome P450 caused strong genetic improvement in early phylogeny of penicillin-producing strains[J].J Bacteriol, 2001,183(19):5465-5471.
[13]  Guerin WF, Boyd SA. Maintenance and induction of naphthalene degradation activity in Pseudomonas putida and an Alcaligenes sp. under different culture conditions[J].Appl Environ Microbiol, 1995, 61(11):4061-4068.
[14]  Del Carratore M R, Mezzatesta C, Hidestrand M. Cloning and expres sion of rat CYP2E1 in Saccharomyces cerevisiae: detection of geno toxicity of N-alkylformamides[J]. Environ Mol Mutagen, 2000, 6 (2):97-104.
[15]  Muller H G,Schunck W H,Kargel E. Cytochrome P450 in alkane -assimilating yeast[A].In:Ruckpaul, K. et al (eds).Microbial and Plant Cytochrome[C]. London:Taylor and Francis, 1991. 87-147.
[16]  Schrenk D, Riebniger D, Till M. Tryptanthrins: a novel class of ago nists of the aryl hydrocarbon receptor[J]. Biochem Pharmacol,1997, 54(1):165-171.

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