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尿酶抑制剂对猪粪氮素水平和挥发性脂肪酸产生量影响的研究

Keywords: 尿素,尿酶抑制剂,,臭气化合物,猪粪

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Abstract:

采用室内培养方法,研究评估了苯磷酸二酰胺(PPDA)、环己基磷酸三胺(CHPT)和N-n-丁基硫代磷酸三酰胺(NBPT)3种尿酶抑制剂对猪粪氨的挥发、恶臭化合物的排放、猪粪的化学成分及猪粪中主要菌群的影响。结果表明,3种尿酶抑制剂都能不同程度地抑制氨的挥发,累积更多的尿素。不添加尿酶抑制剂组尿素在2d内基本被水解完;而PPDA组2d内尿素浓度达到4.9g·kg-1粪,然后逐渐水解,至第10d水解完全;CHPT组4d内尿素浓度达到5.2g·kg-1粪,至第14d仍可检测到少量的尿素;NBPT组8d内尿素浓度达到5.9g·kg-1粪,至14d仍可检测到0.8g·kg-1粪。3种尿酶抑制剂抑制尿素水解减少氨的挥发时,可以使更多的氮素以NH4-N的形式得以保留。PPDA、CHPT和NBPT组的NH4-N分别比对照组增加了4.4%(P<0.01)、21.6%(P<0.01)和26%(P<0.01)。NBPT的使用还能降低猪粪pH值,减少戊酸(37.4%,P<0.01)和异戊酸(50.3%,P<0.01)的含量。就猪粪的主要菌群而言,NBPT能减少梭菌(33.7%,P<0.01)和增加乳酸菌(29.4%,P<0.01)的数量,但对大肠杆菌和优杆菌的数量却无显著影响。

References

[1]  Pain B F,Thompson R B,Rees Y J,et al.Reducing nitrogen losses from cattle slurry applied to grassland by the use of additives [J].Journal of A nimal Science,1990,45:1188-1203.
[2]  Mackie R I,Stroot P G,Varel V H.Biochemical identification and biological origin of key odor compounds in livestock waste [J].Journal of Animal Science,1998,76(5):1331-1342.
[3]  Eghball B,Power J F.Beef cattle feedlot manure mangement[J].Journal of Soil Water Conservation,1994,49:112-113.
[4]  Van Horn H H,Newton G L,Kunkle W E.Ruminant nutrition from environment perspective:factors affecting whole-farm nutrient balance[J].Journal of A nimal Science,1996,74:3082-3102.
[5]  Gill J S,Khind C S.Efficiency of N-(n-butyl) thiophosphoric triamide in retarding hydrolysis of urea and ammonia volatilization losses in a flooded sandy loam soil amended with organic materials [J].Nutrient Cycling in Agroecosystems,1999,53(3):203-207.
[6]  Rideout T C,Fan M I.Efficacy of various microbial urease inhibitors in controlling ammonia and volatile sulfide emissions from manure slurry [J].Journal of Dairy Science,2002,85 (supplement 1):396.
[7]  Shi Y,Parker D B,Cole D A,et al.Surface amendments to minimize ammonia emissions from beef cattle feedlots[J].Transaction of the ASAE,2001,44(3):677-682.
[8]  徐明.尿酶抑制剂在反刍动物生产中的应用[J].兽药与饲料添加剂,2003,8(2):34-36.
[9]  崔九思,王钦源,王汉平.大气污染监测方法(第二版)[M].北京:化学工业出版社,1997.
[10]  李影林.培养基手册[M].长春:吉林科学出版社,1991.
[11]  陈天寿.微生物培养基的制造与运用[M].北京:中国农业出版社,1999
[12]  Varel V H,Nienaber J A,Freetly H C.Conservation of nitrogen in cattle feedlot waste with urease inhibitors [J].Journal of Animal Science,1999,77(5):1162-1168.
[13]  Mobley H L T,Island M D,Hausinger R P.Molecular biology of microbial urease[J].Microbial Rev,1995,59:451-480.
[14]  Zhu J.A review of microbiology in swine manure odor control[J].Agriculture,Ecosystems and Environment,2000,78(2):93-106.
[15]  Zahn J A,Dispiroto A A,Do Y S,et al.Correlation of human olfactory responses to airborne concentrations of malodorous volatile organic compounds emitted from swine effluent[J].J Environ Qual,2001,30:624-634.
[16]  Spoelstra S F.Origin of objectionable odorous components in piggry wastes and the possibility of applying indicator components for studying odour development[J].Agric Env,1980,5:241-260.
[17]  Ouwerkerk D,Klieve A V.Bacterial diversity within feedlot manure[J].Anaerobe,2001,7(2):59-66.
[18]  Cotta M A,Whitehead T R,Zeltwanger R L.Isolation,characterization and comparison of bacteria from swine faces and manure storage pits[J].Environmental Microbiology,2003,5(9):737-745.

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