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吩嗪-1-羧酸降解菌的筛选及其特性研究

Keywords: 吩嗪-1-羧酸,降解菌,分离筛选

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Abstract:

用以吩嗪-1-羧酸为惟一碳氮源的培养基从土壤中分离得到了96株能够降解吩嗪-1-羧酸的菌株。通过限制性内切酶酶切分析(amplifiedribosomalDNArestrictionanalysis,ARDRA),共将其分为16种类型,对这16种类型的代表菌株16SrDNA的部分序列(500bp)进行基因测序,并将测序结果在GenBank中比对,分析其在系统发育分类学上的地位。其中,菌株V-6,204和207分属于细菌拟杆菌门的不同纲目,菌株132属于放线菌中的红球菌属,其他12株菌株分属于α、β、γ-变形菌纲。对16株代表菌株降解吩嗪-1-羧酸的能力进行了测定,结果表明,16株代表菌株能降解吩嗪-1-羧酸,但其降解效率低,培养35d后的降解率为16.5% ̄34.6%。降解效率最高的两株菌株为133和102,其降解率分别为34.6%和32.9%,与菌株133亲缘关系最近的菌为Pseudomonasalcaligenes,同源性为100%;与菌株102亲缘关系最近的菌为PseudomonasputidaKT2440,同源性为99%。而将16株代表菌株人为组成混合微生物体系后,其降解效率7d即可达到26.8%,28d后达到43.8%,混合菌株对吩嗪-1-羧酸的降解效率明显优于单菌。本研究首次在环境中筛选分离到了能够降解吩嗪-1-羧酸的菌株,为进一步研究吩嗪-1-羧酸在环境中的行为提供了可能。

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