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牛血清乙酰胆碱脂酶的分离纯化

Keywords: 牛血清,提纯,胆碱酯酶

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Abstract:

以牛血清为原材料,采用硫酸铵分级沉淀、SephadexG-100凝胶柱和DEAE-纤维素DE23柱层析对牛血清中的乙酰胆碱酯酶进行分离纯化。结果表明,经以上三步分离纯化后,可获得电泳纯的乙酰胆碱酯酶,最终收集的酶液经聚丙烯酰胺(PAGE)凝胶电泳鉴定后,仅出现一条谱带;对SephadexG-100凝胶和SephadexG-75凝胶分离纯化乙酰胆碱脂酶的效果进行了比较,发现两者对乙酰胆碱酯酶的分离效果相差不大。

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