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克百威及其代谢产物对小鼠DNA损伤的研究

Keywords: 克百威,微核,单细胞凝胶电泳,遗传毒性

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Abstract:

采用微核试验和单细胞凝胶电泳技术研究了克百威及其4种主要代谢物的细胞及遗传毒性,对每种受试物设立3个不同剂量组,微核试验以小鼠活体给药后分离骨髓淋巴细胞,单细胞凝胶电泳试验采用分离外周血细胞后受试物处理的方法进行。结果表明,克百威、呋喃酚在两种方法中均显示阴性结果,可认为无明显细胞及分子毒性;3-羟基呋喃丹和亚硝基呋喃丹均表现明显的细胞毒性,而3-酮基呋喃丹虽无明显微核效应,却可引起细胞强烈的DNA单链断裂,后3种化合物可能具有对机体的毒性,需要进一步确定其毒害机制。

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