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氯苯类胁迫对蚕豆幼苗超氧化物歧化物活性的影响

Keywords: 土壤,氯苯类污染,蚕豆,SOD活性,生物标记物

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Abstract:

采用室内培养方法,研究了土壤中不同浓度氯苯(CB)、1,2,4-三氯苯(TCB)和六氯苯(HCB)胁迫对蚕豆幼苗生长、超氧化物歧化酶(SOD,EC1.15.1.1)活性及丙二醛(MDA)含量变化的影响。结果表明,50~300μg·g-1TCB处理5d后蚕豆幼苗生长受到抑制(P<0.05和P<0.01)。50~300μg·g-1TCB胁迫1~5d对蚕豆幼苗SOD活性的影响表现出明显的剂量-效应关系。TCB处理5d后的幼苗恢复培养3d,50μg·g-1TCB处理组的SOD活性得到恢复,而100和300μg·g-1TCB处理组的SOD活性明显高于对照(P<0.05和P<0.01)。试验亦表明,100和300μg·g-1TCB处理3d和5d,幼苗MDA含量均比对照显著增加(P<0.05和P<0.01),并且与TCB浓度之间存在明显的正相关关系。上述结果表明,蚕豆幼苗SOD活性及MDA含量可作为短期土壤TCB污染的生物标记物,而50~1000μg·g-1CB或HCB处理5d对蚕豆幼苗没有产生明显的毒性效应。

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