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氮锌交互作用对白三叶草叶片活性氧代谢和叶绿体超微结构的影响

Keywords: ,,交互作用,白三叶草,活性氧,叶绿体超微结构

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Abstract:

在砂培条件下,研究了不同水平氮锌配施对白三叶草叶片活性氧代谢和叶绿体超微结构的影响。结果表明,在缺锌条件下,随氮水平增加,植株干物重、叶片叶绿素、SOD、类胡萝卜素含量显著下降,MDA含量则明显增加,即白三叶草叶片光合作用和清除活性氧的能力显著下降,氮锌配施在缺锌条件下表现为拮抗效应。在足量供锌条件下,中量供氮,植株干物重、叶片叶绿素、SOD、葫萝卜素含量高于低量供氮,MDA含量则相反,氮锌配施表现为协同效应;与中低水平供氮相比,当高水平供氮时,各组分干物重、叶片叶绿素、SOD、类胡萝卜素含量等显著下降,MDA含量显著增加,氮锌配施表现为拮抗效应。叶片超显微结构表明,低锌条件下,随供氮水平增加,叶绿体结构变差,基粒及基粒片层、基质片层数量下降甚至解体,光合能力显著下降;足量供锌时,以中量供氮叶绿体基粒及基粒片层、基质片层数量最多,内部结构有序性最高,光合能力最强。叶绿体超显微结构变化与叶绿素、SOD、类胡萝卜素含量变化趋势相一致。

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