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除虫脲农药残留的酶联免疫测定方法

Keywords: 除虫脲,免疫吸附检测法,杀虫剂,环境分析

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Abstract:

通过研究除虫脲半抗原的合成、分离纯化方法和多克隆免疫抗体的制备,建立了直接竞争酶联免疫吸附检测法(ELISA)。结果表明,所制备的抗体对除虫脲的特异性好,免疫检测灵敏度高、稳定性好,土壤(90%甲醇萃取)、水样及其他样品基质基本不对检测结果造成影响,对除虫脲的检测限可以达到0.05μg·dm-3。对土壤和水样的添加试验回收率均超过80%。

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