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六氯苯作用下鱼肝线粒体蛋白质表达差异研究

Keywords: 六氯苯,鲫鱼肝脏,线粒体蛋白质,表达差异,SDS-聚丙烯酰胺凝胶电泳

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Abstract:

用差速离心和核酸酶消化法从鲫鱼肝组织中提取和纯化线粒体,用不同浓度(0、10、20、30、40、50mg·L-1)的六氯苯对线粒体进行体外染毒(35℃,2h),并对线粒体蛋白质进行SDS-PAGE分析。结果显示,在一定浓度范围(0~50mg·L-1)内,随着六氯苯浓度的增加,线粒体表达的蛋白质条带呈现先增加后减少的现象,另外多肽B(Mr为63kD)的表达量明显减小甚至消失,表明六氯苯可能引起线粒体内遗传体系发生改变,导致线粒体蛋白质表达出现差异。这一结果为进一步研究六氯苯对线粒体的毒性作用机理具有一定意义,也为在亚细胞水平上研究污染物的毒性效应提供了一种可能的方式。

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