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农业环境科学学报 2007

假单胞菌DS-Ⅲ的脱氢酶活性与石油烃降解动力学特性

Keywords: 石油烃,微生物降解,脱氢酶活性,米氏方程

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Abstract:

通过从油污土壤中分离筛选出的石油烃降解菌假单胞菌DS-Ⅲ,研究了在以烃为惟一碳源条件下该菌的生长特征、降解性与脱氢酶活性变化之间的关系。结果表明,DS-Ⅲ的脱氢酶活性滞后于其细胞生物量增长,适应后DS-Ⅲ的脱氢酶活性变化与柴油的降解趋势基本一致,DS-Ⅲ菌的对数生长期代时为4.05d,生长速率为0.25d-1。在培养温度32℃、pH6.5时,DS-Ⅲ的脱氢酶活性达到最高,在该条件下获得的米氏方程中,Km为159.16μg·L-1,Vmax为11.06μg·L-1·d-1。

References

[1]	贾建丽,李广贺,钟毅.油污土微生态环境非生物因子与微生物活性关系[J].环境科学,2004,25(3):111-114.
[2]	马宏瑞,蔡勇,朱艳萍,等.石油烃污染地下水吸附-降解联合修复方法的研究[J].农业环境科学学报,2006,25(2):145-148.
[3]	Gong Ping.Dehydrogenase activity in soil:a comparison between the TTC and INT assay under their optimum conditions[J].Soil Biol Biochem,1997,29(2):211-214.
[4]	尹军,谭学军,张立国.测定脱氢酶活性的萃取剂选择[J].中国给水排水,2004,20(7):96-98.
[5]	周春生,尹军.TTC-脱氢酶活性检测方法的研究[J].环境科学学报,1996,16(4):400-405.
[6]	臧荣春,夏凤毅.微生物动力学模型[M].北京:化学工业出版社,2003.
[7]	Trasar-Cepeda C.Limitations of soil enzymes as indicators of soil pollution[J].Soil Biology & Biochemistry,2000,32:1867-1875.
[8]	Margesin R,Zimmerbaner A,Schinner F.Monitoring of bioremediation by soil biological activities[J].Chemosphere,2000,40:339-346.
[9]	Neilson A H.Organic chemicals:An environmental perspective[M].New York:Lewis Publishers,2000.
[10]	Marin J A,Hernandez T,Garcia C.Bioremediation of oil refinery sludge by landfarming in semiarid conditions:Influence on soil microbial activity[J].Environmental Research,2005,98:185-195.
[11]	Namkoong W.Bioremediation of diesel-contaminated soil with composting[J].Environmental Pollution,2002,119:23-31.
[12]	李广贺,张旭,卢晓霞.土壤残油生物降解性与微生物活性[J].地球科学-中国地质大学学报,2003,27(2):181-185.

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