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MTBE降解菌的分离及降解动力学分析

Keywords: 甲基叔丁基醚,生物降解,酵母膏,钴离子,降解动力学

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Abstract:

从甲基叔丁基醚(MTBE)污染场地的土壤样品中分离得到一株高效降解菌株A-3,经形态及生理生化鉴定和16SrDNA基因序列分析,该菌株鉴定为金黄杆菌(Chryseobacteriumsp.)。对该菌株降解MTBE的特性进行了研究,结果表明:添加100mg·L-1酵母膏及0.05~0.1mg·L-1Co2+对细菌的生长及MTBE的降解具有一定的促进作用。对该菌的降解动力学进行了分析,MTBE的降解符合抑制动力学Haldane模型,最大降解速率Vmax=0.11d-1,半饱和常数ks=161.7mg·L-1,抑制常数k1=68.2mg·L-1。该菌株对于烃类等物质具有广泛的利用能力,其在环境中的应用具有一定的意义。

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