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Zn~(2+)胁迫对小麦幼苗生理活性影响的研究

Keywords: Zn2+,小麦,幼苗,生理活性

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Abstract:

以郑州9023为供试材料,采用室内水培方法,研究了不同浓度Zn2(+0、30、60、120、240、360mg·L-1)对小麦幼苗生理活性的影响,初步阐明了Zn2+对小麦幼苗的毒害机理。结果表明,在Zn2+胁迫下,随处理浓度的升高,小麦幼苗叶片中超氧阴离子自由基(O·2-)含量、丙二醛(MDA)含量、可溶性糖含量、抗坏血酸过氧化物酶(APX)及过氧化物酶(POD)活性均呈上升趋势,超氧化物歧化酶(SOD)活性呈先下降后上升的趋势;抗坏血酸(ASA)含量、过氧化氢酶(CAT)活性均呈现先上升后下降的趋势,可溶性蛋白和叶绿素含量呈下降趋势。对SOD、POD、CAT同工酶的聚丙烯酰胺凝胶电泳结果分析表明,正常情况下,小麦幼苗叶片主要表达Rf值(相对迁移率)分别为0.98、0.96、0.9、0.8、0.6、0.48的6条SOD同工酶带;POD同工酶带主要分为5区,至少包括10条同工酶酶带;CAT主要表达2条同工酶带。浓度小于120mg·L-1的Zn2+抑制SOD同工酶的表达,促进CAT同工酶的表达,浓度大于240mg·L-1的Zn2+促进SOD同工酶的表达,抑制CAT同工酶的表达,Zn2+诱导小麦幼苗中POD同工酶的表达。Zn2+没有诱导新的CAT、SOD和POD同工酶的表达。

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