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4种菌根真菌对五氯酚耐受性及其生理基础研究

Keywords: 外生菌根真菌,五氯酚,氧化还原酶,pH,点试

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Abstract:

通过纯培养的方法,研究了4种外生菌根真菌——美味牛肝菌(Boletusedulis)、褐环乳牛肝菌(Suillusluteus)、丝膜菌、(Cortinarrusrussus)和厚环粘盖乳牛肝菌(Suillusgrevillei)的生长效应、对五氯酚的耐受性,并采用点试方法对真菌氧化还原酶的产生情况进行了检测。结果表明,4种外生菌根真菌在MM培养基上的生长速率显著高于MMN培养基。4种外生菌根真菌对五氯酚均有一定的耐受性,厚环粘盖乳牛肝菌(Suillusgrevillei)耐受性最高,其生长抑制率显著低于其它真菌。酶测试结果表明,厚环粘盖乳牛肝菌是4种真菌中氧化还原酶活性较强且酶种类多样的菌株,它表现出很高的过氧化物酶、多酚氧化酶和漆酶活性,是一种具有高效降解芳环结构污染物潜力的菌根真菌。4种真菌在生物量和“酸化效应”方面差异较大。厚环粘盖乳牛肝菌(Suillusgrevillei)对五氯酚的高耐受性可能与其产酶特性和“酸化效应”有关。

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