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外加肌醇和氯化钠对嗜鞣管囊酵母生长和产酒精能力及耐酒精能力的影响

Keywords: 嗜鞣管囊酵母,发酵,肌醇,氯化钠,耐酒精

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Abstract:

嗜鞣管囊酵母(Pachysolentannophilus)是可以同时发酵葡萄糖和木糖为酒精的菌种,在其培养基中分别添加不同浓度的(0~0.2g·L-1)肌醇和(0~1.5g·L-1)NaCl,以考察它们对嗜鞣管囊酵母生长、产酒精能力和耐酒精能力的影响。结果表明,外加肌醇和NaCl对嗜鞣管囊酵母生长有轻微的刺激作用,而对酵母生长的耐酒精能力却有明显的影响,并且,菌种在YEPD培养基中的耐酒精能力高于在YEPX培养基中的耐酒精能力。经实验测定,肌醇和NaCl对嗜鞣管囊酵母产酒精能力及发酵的耐酒精能力均有显著的影响。当发酵培养基中未添加酒精,肌醇和NaCl浓度各为0.1g·L-1和1.5g·L-1时,可以分别达到的最高酒精产量为45.20g·L-1和40.12g·L-1。在发酵过程中,当酒精起始浓度分别为10%和12%时,随肌醇浓度的增加,酒精的净生成量呈上升趋势,最高分别为17.18g·L-1和16.68g·L-1;添加NaCl后,酒精的净生成量亦呈现相同的规律,最高可达10.16g·L-1和9.87g·L-1。

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