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Cd、Pb复合胁迫下湿地匍灯藓(Plagiomnium acutum)重金属累积效应及抗氧化系统响应

DOI: 10.11654/jaes.2015.05.005

Keywords: 湿地匍灯藓(Plagiomnium acutum) Cd、Pb复合胁迫 累积效应 抗氧化系统 响应

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Abstract:

采用浸没培养方法研究了不同浓度Cd、Pb复合胁迫下湿地匍灯藓(Plagiomnium acutum)重金属的累积效应及抗氧化系统的响应。结果显示:复合胁迫下Pb显着抑制湿地匍灯藓对Cd的吸收,与低 Pb 水平(20 mg·L-1)相比(对照无检出值),在低 Cd(2 mg·L-1)处理时,随Pb 处理浓度增加,藓体内 Cd 累积量分别降低了30.1%和 78.4%;在高 Cd(50 mg·L-1)水平处理时,其降幅则分别达到了 35.0%和 86.9%.复合胁迫引起湿地匍灯藓脂质过氧化终产物丙二醛(MDA)含量随胁迫程度加大而持续升高,当复合胁迫浓度达到最大时,MDA含量也达到了最大,与对照相比显着增加7162%.与此同时,超氧化物歧化酶(SOD)活性和脯氨酸含量也呈现了应激性增加,且均在最高复合胁迫浓度处理时达到最大,与对照相比分别增加237.4%和2049%,而过氧化氢酶(CAT)和过氧化物酶(POD)活性则持续降低。基于此,可以认为:Cd、Pb复合胁迫下,Pb对Cd的累积效应有明显的抑制作用,且这种抑制作用有浓度依赖性;Cd、Pb复合胁迫所致细胞氧化损伤的主要来源为过氧化物的清除能力低下所致过氧化物积累

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