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土壤中Cd2+胁迫对gfp基因标记的饭豆(Vigna umbellate L.)根瘤菌生物毒性的研究

Keywords: 饭豆,根瘤菌,Cd,生物毒性,土壤

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Abstract:

利用绿色荧光蛋白基因(gfp)标记研究了土壤中Cd2+胁迫对饭豆(VignaumbellateL.)根瘤菌JMC1402G腐生存活的影响,同时也探讨了Cd2+胁迫对其共生固氮能力的影响。结果表明,YMA培养基平板培养时,Cd2+对JMC1402G的LC50为8.15μg·mL-1。不管土壤灭菌与否,当土壤中Cd2+添加量为3mg·kg-1时,向土壤接种的37d内对JMC1402G的腐生存活无影响,当Cd2+添加量为20mg·kg-1时,土壤中JMC1402G的数量明显低于对照,随着Cd2+添加量的进一步增大,土壤中JMC1402G的数量迅速落于104cfu·g-1以下。饭豆盆栽试验结果表明,Cd2+添加量在0~20mg·kg-1范围内对JMC1402G共生固氮能力的影响不大,当土壤中Cd2+添加量达到50mg·kg-1时,对JMC1402G产生较明显的生理毒性,当Cd2+添加量达到100mg·kg-1,饭豆根瘤菌-饭豆体系失去结瘤能力。JMC1402G质粒快检结果表明,JMC1402G在Cd2+添加量为0、3、20、50、100mg·kg-1的土壤中营腐生生活37d,未出现质粒丢失情况。

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