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不同铜水平下玉米细胞内铜的分布和化学形态的研究

Keywords: 玉米,,亚细胞分布,化学形态

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Abstract:

运用差速离心法和化学试剂逐步提取法分析了铜在玉米细胞内的分布和存在的主要化学形态。结果表明,从含量上看,细胞壁和细胞溶质部分是铜在玉米细胞内分布的主要位点,细胞核、叶绿体及线粒体等细胞器中铜的含量较低。对照和1μmol·L-1Cu处理的玉米根部中,铜主要分布在细胞壁区域,其次为细胞溶质部分。随着铜浓度的升高,铜向细胞壁部分的分配减少,而向细胞溶质部分的分配增加。在茎中,就不同铜浓度处理来说,亚细胞组分中铜的含量均以细胞溶质部分的值最高,其次为细胞壁部分。而在叶中,均以细胞壁部分的值最高,其次为细胞溶质部分。就铜的存在形态而言,对照处理的根部主要以HCl提取态铜为主,茎和叶中铜以多种化学形态存在。1μmol·L-1Cu处理时,根部铜仍以HCl提取态为主,而茎和叶中以NaCl提取态占优势。随着铜浓度的升高,根部和叶部的铜均主要以乙醇提取态为主,而茎中20和50μmol·L-1铜处理主要以NaCl提取态铜为主,80μmol·L-1Cu处理时主要以去离子水提取态铜和NaCl提取态铜为主。

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