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抗DDA抗体的制备及其特性研究

Keywords: DDA,DDT,抗DDA抗体,酶联免疫吸附实验

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Abstract:

制备抗DDA抗体,建立间接竞争酶联免疫吸附实验(IndrectCompetitiveEnzyme-linkedImmunosorbentAssay,idc-ELISA)方法,以测定海水样品和海洋贝类中的有机氯农药滴滴涕(DDT)代谢产物。采用碳二亚胺法,将半抗原DDA分别与载体蛋白牛血清白蛋白(Bovineserumalbumin,BSA)和卵清蛋白(Ovalbumin,OVA)偶联,得到完全抗原。以DDA-BSA为抗原,免疫BALB/c小鼠,制备抗DDA抗体。研究表明,5只鼠产生的抗体对DDA都能产生灵敏的特异性免疫反应,以DDA-OVA为包被抗原,用5#抗体建立间接竞争ELISA方法检测海水样品和海洋贝类中的有机氯农药DDT代谢产物DDA,最低检出限为:10.0ng·mL-1,海水加标样平均回收率122%,批间变异系数30%,贝类加标样平均回收率为131%,批间变异系数22%。

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