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二氧化硫暴露对拟南芥叶片形态和生理生化指标的影响

Keywords: 拟南芥,SO2,生长发育,叶片形态,抗氧化酶

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Abstract:

采用室内培养及密闭箱静态熏气方法,研究了不同浓度SO2暴露对拟南芥(Arabidopsisthaliana)叶片形态和生理生化指标的影响。结果显示,拟南芥暴露于SO214d后,叶面气孔开度变小。浓度10mg·m-3组促进植株生长发育,SOD和CAT活性无明显改变,30mg·m-3SO2组3种酶活性呈诱导性增高,其中POD增幅最大,SO2浓度90mg·m-3时,CAT活性被抑制,丙二醛(MDA)含量增加,株高、叶片数和叶片面积显著降低,可溶性蛋白含量减少,叶片出现明显可见伤害。研究结果表明,在低浓度条件下,拟南芥能够通过调节气孔开放、细胞抗氧化酶活性等使植株适应SO2胁迫,但是高浓度SO2暴露会引起细胞氧化损伤,影响植物生长发育。

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