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离子液体1-辛基-3-甲基咪唑溴化盐对小麦种子萌发与幼苗生长的影响

Keywords: 离子液体,冬小麦,种子萌发,幼苗生长,抑制效应

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Abstract:

用不同浓度(0、1、2、4、6、8mg·L-1)的1-辛基-3-甲基咪唑溴化盐(C8mimBr)对冬小麦进行浸种和苗期水培,测定了种子的发芽势(3d)、发芽率(7d)和淀粉酶活力以及幼苗根长、芽长、根活力、超氧阴离子(O2-)产生速率、叶片光合色素和脂质过氧化产物丙二醛(MDA)含量(7d)。结果表明,离子液体的暴露导致小麦种子发芽势、发芽率和淀粉酶活力均比对照显著降低,其IC50分别为5.55、3.39、4.02mg·L-1;同时,处理组幼苗根长、芽长、根活力以及叶片中叶绿素a、叶绿素b、总叶绿素和类胡萝卜素含量也显著下降,其对幼苗根与苗生长的IC50分别为0.64mg·L-1和4.98mg·L-1。另外,处理组幼苗叶片中超氧阴离子产生速率和丙二醛含量均高于对照,差异多达显著或极显著水平,分别为对照的113.63%~224.15%和101.69%~107.61%。在本试验条件下,1-辛基-3-甲基咪唑溴化盐对小麦种子萌发和幼苗生长均具有明显的抑制效应,且抑制效应具有典型的剂量依赖型特点。

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