全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

铜胁迫对苜蓿中华根瘤菌抗氧化酶系的影响

Keywords: 苜蓿中华根瘤菌,铜胁迫,抗氧化酶

Full-Text   Cite this paper   Add to My Lib

Abstract:

探讨铜胁迫对苜蓿中华根瘤菌抗氧化酶系的影响,揭示苜蓿中华根瘤菌对铜的生理抗性机制。以铜抗性菌株SinorhizobiummelilotiCCNWSX0020和铜敏感性S.melilotiCCNWSX0018为材料,测定其对铜的最小抑制浓度(MIC)和最大耐受浓度(MTC)及不同铜浓度对其抗氧化保护酶活性的变化。结果表明:(1)在YMA固体培养基上,S.melilotiCCNWSX0020和S.melilotiCCNWSX0018的MIC分别为0.5mmol·L-1和0.2mmol·L-1Cu2+,MTC分别为1.8mmol·L-1和0.8mmol·L-1Cu2+。(2)Cu2+浓度≤0.4mmol·L-1时,S.melilotiCCNWSX0020菌体内的SOD、CAT和GPX活性变化不显著;S.melilotiCCNWSX0018菌体内的SOD、CAT和GPX活性显著升高;Cu2+浓度为0.6mmol·L-1和0.8mmol·L-1时,前者SOD、CAT和GPX活性显著升高,后者保护酶活性开始降低。随着Cu2+浓度升高,S.melilotiCCNWSX0020的GR活性增强,与对照相比,Cu2+浓度为0.8mmol·L-1时GR活性提高了110.51%;而S.melilotiCCNWSX0018的GR活性则反之。(3)在Cu2+浓度≤0.8mmol·L-1胁迫下,抗性菌株S.melilotiCCNWSX0020可通过提高SOD、CAT、GPX、GR的活性以降

References

[1]  Merkamm,M ;Guyonvarch,A,Cloning of the sodA gene from Corynebacterium melassecola and role of superoxide dismutase in cellular viability.,Journal of Bacteriology?,2001, 183(4).
[2]  Sierra-Campos E;Pardo J P,The relationship between the antioxidant system and the virulence in Ustilago maydis,a fungal pathogen,Reviews Latinoam Microbiology,2009(1-2).
[3]  Aguirre J ;Rios-Momberg M ;Hewitt D ;Hansberg W,Reactive oxygen species and development in microbial eukaryotes,Trends in microbiology?,2005, 13(3).
[4]  Dalton DA;Russell SA;Hanus FJ;Pascoe GA Evans HJ,Enzymatic reactions of aseorbate and glutathione that prevent peroxide damage in soybean root nodules,Proceedings of the National Academy of Sciences(USA)? ,1986, 83.
[5]  Bhattacharyya S;Pal T K;Basumajumdar A,Modulation of enzyme activities of a lead-adapted strain of Rhizopus arrhizus during bioaccumulation of lead,Folia Microbiologica? ,2009, 54(06).
[6]  Beers R F;Sizer I W,A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase,Journal of Biological Chemistry,1952.
[7]  Beauchamp C;Fridovich I,Superoxide dismutase:improved assays and an assay applicable to acrylamide gels,Analytical Biochemistry? ,1971, 44(01).
[8]  Fan L M;Ma Z Q;Liang J Q,Characterization of a copper-resistant symbiotic bacterium isolated from Medicago lupulina growing in mine tailings,Bioresource Technology? ,2011, 102(02).
[9]  Balestrasse KB ;Gallego SM ;Tomaro ML,Cadmium-induced senescence in nodules of soybean (Glycine max L.) plants,Plant and Soil?,2004, 262(1/2).
[10]  Scandalios J G,Oxygen stress and superoxide dismutases,Plant Physiology,1993.
[11]  Monk L S;Fagerstedt K V;Crawford R M M,Oxygen toxicity and superoxide dismutase as antioxidant in oxidative stress,Physiologia Plantarum,1989.
[12]  Obbard JP.,Ecotoxicological assessment of heavy metals in sewage sludge amended soils,Applied Geochemistry?,2001, 16(11/12).
[13]  Ibekwe A M;Angle J S;Chaaney R L,Sewage sludge and heavy metal effects on nodulation and nitrogen fixation legumes,Journal of Environmental Quality? ,1995, 24.
[14]  Brenot A;King KY;Janowiak B,Contribution of glutathione peroxidase to the virulence of Streptococcus pyogenes,Infection and Immunity? ,2004, 72(01).
[15]  Sigaud,S ;Becquet,V ;Frendo,P ;Puppo,A ;Herouart,D,Differential regulation of two divergent Sinorhizobium meliloti genes for HPII-like catalases during free-living growth and protective role of both catalases during symbiosis.,Journal of Bacteriology?,1999, 181(8).
[16]  Athina A;Eddy J S;Marjon H J B,Antioxidative properties of Lactobocillus sake upon exposure to elevated oxygen concentrati-ons,FEMS Microbiology Letters,2001.
[17]  Figueira, E M de A P ;Lima, A I G ;Pereira, S I A,Cadmium tolerance plasticity in Rhizobium leguminosarum bv. viciae: glutathione as a detoxifying agent.,Canadian journal of microbiology?,2005, 51(1).
[18]  缪福俊;熊智;李素停.会泽铅锌尾矿区豆科植物根瘤菌耐铅锌性及其生理生化特征研究[J].农业环境科学学报,2010(10)
[19]  Gomathy M;Sabarinathan K G,Microbial mechanisms of heavy metal tolerance,Agricultural Reviews,2010(2).
[20]  Silver S;Phungle T,A bacterial view of the periodic table:Genes and proteins for toxic inorganic ions,Journal of Industrial Microbiology and Biotechnology,2005.
[21]  Nies DH.,Efflux-mediated heavy metal resistance in prokaryotes [Review],FEMS Microbiology Reviews?,2003, 27(2/3).
[22]  Bruins,MR ;Kapil,S ;Oehme,FW,Microbial resistance to metals in the environment.,Ecotoxicology and Environmental Safety?,2000, 45(3).
[23]  M.M.Posmyk ;R.Kontek ;K.M.Janas,Antioxidant Enzymes Activity And Phenolic Compounds Content In Red Cabbage Seedlings Exposed To Copper Stress,Ecotoxicology and Environmental Safety?,2009, 72(2).
[24]  Schickler, H ;Caspi, H,Response of antioxidative enzymes to nickel and cadmium stress in hyperaccumulator plants of the genus Alyssum,Physiologia plantarum?,1999, 105(1).
[25]  Stochs S J;Bagchi D,Oxidative mechanism in the toxicity of metal ions,Free Radical Biology and Medicine,1995.
[26]  Corticheiro S C;Lima A I G;Figueira E M A P,The importance of glutathione in oxidative status of Rhizobium leguminosarum biovar viciae under Cd exposure,Enzyme and Microbial Technology,2006.
[27]  Singh R;Tripathi R D;Dwivedi S,Lead bioaccumulation potential of an aquatic macrophyte Najas indica are related to antioxidant system,Bioresource Technology,2010.
[28]  Riccillo,PM ;Muglia,CI ;de,Bruijn-FJ ;Roe,AJ ;Booth,IR ;Aguilar,OM,Glutathione is involved in environmental stress responses in Rhizobium tropici, including acid tolerance.,Journal of Bacteriology?,2000, 182(6).
[29]  韦革宏;马占强.根瘤菌-豆科植物共生体系在重金属污染地修复中的地位、应用及潜力[J].微生物学报,2010(11)

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133