全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

水稻耐1,2,4-三氯苯胁迫基因型的苗期筛选

Keywords: 水稻,基因型,TCB胁迫,耐性评定

Full-Text   Cite this paper   Add to My Lib

Abstract:

为了评定不同水稻基因型对1,2,4-三氯苯(TCB)的耐性强弱,确定合适的筛选指标和筛选浓度,在人工气候箱条件下,采用发芽试验和砂培水稻幼苗的方法,对幼苗期水稻基因型耐TCB胁迫的差异性进行了鉴定,并确定了水稻基因型耐TGB胁迫合适的筛选指标和筛选环境。结果表明,以0.35mmol·kg-1沙浓度TCB更适合用于水稻基因型的耐性鉴定。以发芽势、发芽率、最长根长、根数、株高、地上部干重、根干重和总干重的性状相对值(处理测定值/对照测定值×100%)作为幼苗耐性指数(toleranceindex,TI),其中相对最长根长、相对株高、相对地上部分干重、相对根干重和总干重是评价水稻对TCB胁迫耐性强弱的较好指标。基于耐性指数对各基因型进行聚类分析,将50个水稻基因型聚为耐性、较耐、较敏感和敏感4类。

References

[1]  周霞,余刚,张祖麟,等.北京通惠河水和表层沉积物中氯苯类有机物污染现状[J].环境科学,2005,26(2):117-120.ZHOU X,YU G,ZHANG Z L,et al.Chlorobenzenes in water and sur-face sediments from Tonghui River of Beijing[J].Environrnental Science,2005,26(2):117-120
[2]  周霞,余刚,黄俊,等.北京东南郊化工区土壤和植物中氯苯类有机物的残留及分布特征[J].环境科学,2007,28(2):249-254.ZHOU X,YU G,HUANG J,et al.Residues and distribution characters of Chlorobonzenes in soil and plants from Beijing southeast chemical in-dustry zone[J].Environmental Science,2007,28(2):249-254.
[3]  Jaime D,Manuel R,Mario D.1,2,4-Trichlorobonzeue flow characteris-tics in saturated homogeneous and stratified porous media[J].Water Air and Soil Pollution,2006,177:3-17.
[4]  蔡全英,莫测辉,吴启堂,等.部分城市污泥中氯苯类化合物的初步研究[J].环境化学,2002,21(2):139-143.CAI Q Y,MO C H,WU Q T,et al.Preliminary study on the content of chlorobenzenes in selected municipal sludge of Chins[J].Environmental Chemistry,2002,21(2):139-143.
[5]  Brousbach F R,Reineke W.Degradation of chlorobonzenes in soil slurry by a specialized organism[J].Appl Microbiol Biotechnol,1994,42:415-420.
[6]  Freitag D,Ballborn L,Geyer H,et al.Environmental hazard profile of organic chemicals[J].Chemosphere,1985,14:1589-1616.
[7]  Yaowu H,Tieheng S,Ziqing O,et al.Fate of 1,2,4-Trichlorobenzene (1,2,4-TCB) in soil-flee paddy system[J].Chemosphere,1996,32 (7):1381-1389.
[8]  王泽港,万定珍,杨亚春,等.1,2,4-三氯苯和萘对水稻产量及品质的影响[J].中国水稻科学,2006.20(3):295-300.WANG Z G,WAN D Z,YANG Y C,et al.Effects of 1,2,4-trichloroben and naphthalene on grain yield and quality of rice[J].Chinese Journal of Rice Science,2006,20(3):295-300.
[9]  Jianying Z,Wei Z,Jun P,et al.Tissue-dependent distribution and accu-mulation of chlorobenzenes by vegetables in urban area[J].Environment International,2005,31:855-860.
[10]  Vaghef H,Hellman B.Demonstration of chlorobenzene-induced DNA damage in mouse lymphocytes using the single cell gel electrophoresis assay[J].Toxicology,1995,96(1):19-28.
[11]  Major J,Kemeny G,Tompa A.Genotoxic effects of occupational expo-sure in the perip blood lymphocytes of pesticide preparing workers in Hungary[J].Acta Med Hung,1992-93,49(1-2):79-90.
[12]  Greim H.Mechanistic and toxico kinetic data reducing uncertainty in risk assessment[J].Toxicol Lett,2003,138(1-2):1-8.
[13]  Den Besten C,Smink M C,De Vries E J.Metabolic activation of 1,2,4-trichlorobenzene and pentachlorobenzene by rat liver microsomes:a major role for qninone metabolites[J].Toxicol Appl Pharmacol,1991,108(2):223-233.
[14]  Gallard H,De Last J.Kinetics of oxidation of chlorobenzenes and phenyl-ureas by Fe(Ⅱ)/H2O2 and Fe(Ⅲ)/H2O2.Evidence of reduction and oxidation reactions of intermediates by Fe(Ⅱ) or Fe(Ⅲ)[J].Chemosphere,2001,42(4):405-413.
[15]  Schroll R,Brahushi F,Dorfler,et al.Biomineralisation of 1,2,4-tri-cborobenzene in soils by an adapted microbial population[J].Environ-mental Pollution,2004,127:395-401.
[16]  徐晓白,戴树桂,黄玉瑶.典型化学污染物在环境中的变化及生态效应[M].北京:科学出版社,1998.55-165.XU X B,DAIS G,HUANG Y Y.The change and its ecological effects of typical chemistry pollutant[M].Beijing:Science Press,1998.55-165.
[17]  Kong F X,Hu W,Liu Y.Molecular structure and biochemical toxicity of four halogeno-benzenes on the unicellular green alga[J].Environ Ex-per Bot,1998,40:105-111.
[18]  刘宛,宋玉芳,周启星,等.氯苯胁迫对小麦种子发芽和幼苗生长的影响[J].农业环境保护,2001,20(2):65-68.LIU W,SONG Y F,ZHOU Q X,et al.Effect of chlorobonzene-stress on seed germination and Seedling Growth of Wheat[J].Agro-environmen-tal Protection,2001,20(2):65-68.
[19]  杜青平,贾晓珊,袁保红.1,2,4-TCB对水稻种子萌发及幼苗生长的毒性机理[J].应用生态学报,2006,17(11):2185-2188.DU Q P,JIA X S,YUAN B H.Toxic effects of 1,2,4-trichlorobenzene on rice seed germinationand seedling growth[J].Chinese Journal of Applied Ecology,2006,17(11):2185-2188.
[20]  王书子,吴少辉,高海涛,等.旱地小麦品种筛选鉴定及其形态特征探讨[J].干旱地区农业研究,2001,19(2):76-80.WANG S Z,WU S H,GAO H T,et al.Study on selecting and identifing method and characteristics of dryland wheat varieties[J].Agricultural Research in the Arid Area,2001,19(2):76-80.
[21]  兰居生,胡顺福,张景瑞.作物抗旱指数的概念和统计方法[J].华北农学报,1990,5(2):20-25.LAN J S,HU S F,ZHANG J R.The concept and statistical resistance index method of drought in crops[J].Acts Agriculturae Boreali-Sinica,1990,5(2):20-25.
[22]  冀天会,张灿军,谢惠民,等.小麦品种抗旱性鉴定产量指标的比较研究[J].中国农学通报,2006,22(1):103-106.JI T H,ZHANG C J,XIE H M,et al.A comparative study on yield in-dex of wheat varieties drought resistance[J].Chinese Agricultural Sci-ence Bulletin,2006,22(1):103-106.
[23]  肖美秀,林文雄,陈冬梅,等.耐Cd水稻种质资源的筛选[J].福建农林大学学报(自然科学版),2006,35(2):117-122.XIAO M X,LIN W X,CHEN D M,et al.Selection of rice germplasm tolerant to cadmium stress[J].Journal of Fujian Agriculture and Forestry University (Natural Science Edition),2006,35(2):117-122.
[24]  郭再华,贺立源,徐才国,等.水稻耐低磷种质资源的筛选鉴定指标[J].作物学报,2005,31(1):65-69.GUO Z H,HE L Y,XU C G,et al.Indexes for screening and identify of rice tolerance to low-P stress[J].Acts Agronomics Sinica,2005,31 (1):65-69.
[25]  唐启义,冯明光.实用统计分析及其DPS数据处理系统[M].北京:科学出版社,2002.332-335.Tang Q Y,Feng M G.DPS dataprocessing system for practical statis-tics[M].Boiling:Science Press,2002.332-335.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133