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西北地区金属尾矿地根瘤菌的重金属抗性及其系统发育研究

Keywords: 根瘤菌,重金属,抗性,16S,rDNA

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Abstract:

对分离自陕西、甘肃金属尾矿废弃地寄主为刺槐、鸡眼草、草木樨等23种豆科植物的188株根瘤菌进行了7种重金属的抗性分析、最大抗性水平(MRL)的确定和抗性菌株的系统发育研究。结果表明,菌株之间对重金属的耐受性存在较大差异,大部分菌株表现出对Hg2+、Cd2+、Cr6+(<0.5mmol·L-1)敏感,而对Pb2+(<2.5mmol·L-1)不敏感。在液体培养基中,CCNWSX0403和CCNWSX0360可耐受4.0mmol·L-1Zn2+,分别可耐受2.4mmol·L-1和2.8mmol·L-1Cu2+,CCNWGS0139可耐受0.4mmol·L-1Hg2+,CCNWSX0003可耐受2.4mmol·L-1Ni2+,CCNWGS0284和CCNWGS0142可耐受4.8mmol·L-1Pb2+。9株抗性菌株的16SrDNA全序列分析表明:CCNWGS0122和CCNWSX0003分别属于中慢生根瘤菌属(Mesorhizobium)和中华根瘤菌属(Sinorhizobium);4株对Pb2+、Hg2+耐受性较强的菌株CCNWSX0386、CCNWGS0139、CCNWGS0284和CCNWGS0142均属于土壤杆菌属(Agrobacterium);而属于慢生根瘤菌属(Bradyrhizobium)的3株菌CCNWGS0309、CCNWSX0403和CCNWSX0360对Cu2+、Zn2+耐受性较好。总体上Agrobacterium对Hg2+和Pb2+的耐受性较好,Bradyrhizobium比Rhizobium、

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