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阿特拉津降解菌ADH-2的分离、鉴定及其特性研究

Keywords: 阿特拉津,降解,ADH-2,降解相关基因

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Abstract:

从长期施用阿特拉津的玉米地中采集土样,通过富集培养的方法分离出一株能以阿特拉津为唯一碳、氮源生长的细菌ADH-2,结合生理生化特性及16SrRNA基因的相似性分析将其初步鉴定为节杆菌属(Arthrobactersp.)。该菌在10h内对100mg·L-1阿特拉津的降解率为99.9%。外加氮源能促进菌株的生长,但对阿特拉津的降解有轻微的抑制作用。外加蔗糖和葡萄糖能显著促进菌株的生长,但对阿特拉津的降解表现出显著的抑制。而淀粉既能促进菌株的生长又能促进阿特拉津的降解。对其降解基因的初步研究显示,该菌含有trzN、atzB和atzC?3个阿特拉津降解相关基因。通过与本实验室另外两株阿特拉津降解菌比较,菌株ADH-2具有更好的应用潜力。

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