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丁草胺降解菌的分离鉴定及降解特性的研究

Keywords: 丁草胺,施氏假单孢菌,生物降解

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Abstract:

从处理农药生产废水的膜生物反应器中分离到一株能以丁草胺为惟一碳源和能源生长的细菌BD-1,经鉴定为施氏假单孢菌(Pseudomonasstutzeri)。在纯培养的条件下测定了BD-1对丁草胺的降解性能。结果表明,在接种量为菌浓度OD415=0.2,pH7.0、30℃条件下,BD-1对丁草胺的降解符合一级动力学特征,1.0、10.0和100.0mg·L-1的丁草胺的降解半衰期分别为0.11、0.60和0.96d。BD-1在不同pH及温度下对丁草胺的降解作用为pH7.0>pH6.0>pH8.0,30℃>20℃>40℃。GC/MS初步分析结果表明,丁草胺的主要微生物降解产物为2-氯-2’,6’-二乙基乙酰苯胺和2,6-二乙基苯胺。

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