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外源抗坏血酸对番茄自毒作用的缓解效应

DOI: 10.11654/jaes.2015.07.004

Keywords: 番茄 基质浸提液 抗坏血酸(AsA) 自毒作用 种子萌发 超微结构

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Abstract:

为探究外源抗坏血酸(AsA)提高自毒作用下番茄抗逆能力的内在机制,以优良番茄品种“粉冠一号”为材料,用连作3 a番茄的基质浸提液处理种子及幼苗,研究了不同浓度(0、0.25、0.5、1 mmol·L-1)AsA对自毒作用下番茄种子发芽率、发芽势、发芽指数和幼苗主根、上胚轴长、抗氧化酶(SOD、POD、APX)活性、抗氧化剂(AsA、GSH)含量、MDA含量和电解质渗漏率及根系超微结构的影响。结果表明:单一基质浸提液处理可抑制番茄种子的萌发和幼苗生长,降低SOD、POD和APX酶活性及GSH和AsA含量,MDA含量和电解质渗漏率升高;当用含有不同浓度AsA的基质浸提液处理后,与单一基质浸提液处理相比,番茄种子的发芽率、发芽势和发芽指数均增大,幼苗主根和上胚轴的生长加快,植株的抗氧化酶活性和抗氧化剂含量升高,MDA含量和电解质渗漏率均降低,对幼苗根系结构的不良影响减少。外源AsA能维持番茄幼苗正常的生理功能,有效缓解连茬基质浸提液对其造成的自毒胁迫。所有处理各指标的综合隶属函数值分析表明,1 mmol·L-1的AsA缓解效果最好,0.5 mmol·L-1次之

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