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高/低Cd累积苋菜品种金属累积特性与关键转运基因表达的关系

DOI: 10.11654/jaes.2015.06.004

Keywords: 苋菜 镉累积 IRT1 HMA4 品种差异

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Abstract:

采用受污染菜园土进行盆栽试验, 研究对Cd累积特性不同的苋菜品种(Amaranth mangostanus L.)对金属的吸收差异。白骨小圆叶油苋菜及正泰小圆叶苋菜地上部分Cd含量分别为(0.116±0.007)mg·kg-1和(0.146±0.002)mg·kg-1,台湾白苋菜及红柳叶苋菜地上部分Cd含量分别为(0.210±0.005)mg·kg-1和(0.349±0.026)mg·kg-1,相比而言前两者属Cd低累积品种, 后两者属Cd高累积品种。由于红柳叶苋菜与白骨小圆叶油苋菜Cd含量差异最大, 可通过测定两者根系IRT1及HMA4基因表达量, 以研究高/低Cd累积特性苋菜品种对金属吸收的差异及其与相关转运蛋白的关系。结果显示, 苋菜根系共质体Cd与Fe、Pb的吸收呈极显着正相关(P<0.01).红柳叶苋菜根系原生质体Fe、Cd及Pb含量显着高于白骨小圆叶油苋菜, 同时其根系IRT1表达量高于白骨小圆叶油苋菜, 表明苋菜根系IRT1基因的较高表达有助于根系从土壤吸收Fe外, 可能有助于Cd与Pb的累积。苋菜地上部分Cd与Zn、Fe的吸收呈显着正相关(P<0.05),与Pb、Mn、Ca的吸收呈极显着正相关。红柳叶苋菜地上部分Ca、Fe、Mn、Cd、Pb的含量显着高于白骨小圆叶油苋菜, 同时其根系HMA4基因表达量高于白骨小圆叶油苋菜, 表明苋菜HMA4基因的较高表达可能有利于苋菜将根系吸收的Cd等金属转运至茎叶

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