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养殖废水中异养硝化细菌的分离 筛选和鉴定

Keywords: 养猪场废水,异养硝化细菌,分离,脱氮能力,粪产碱杆菌

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Abstract:

为了获得脱氮功能强的异养硝化菌株用于养殖废水的脱氮处理,通过富集、分离和纯化等步骤,并结合格利斯试剂检验菌株硝化能力的方法,从某养猪场污水处理池污泥中分离筛选了4株具异养硝化功能的菌株,分别标号为79、84、L116、L117,通过16SrDNA序列分析和美国全自动微生物分析仪Biolog鉴定,4株菌均为粪产碱杆菌(Alcaligenesfaecalis),并验证了这4株菌的硝化能力。结果表明,当液体培养基初始氨氮浓度为90mg·L-1左右时,在振荡培养48h内,菌株79、84、L116、L117培养基中氨氮和总氮均快速下降,氨氮去除率分别达到44.4%、47.9%、61.3%和56.4%,总氮(除菌)去除率达到39.9%、38.5%、43.4%和40.7%。

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