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马拉硫磷对双齿围沙蚕乙酰胆碱酯酶和过氧化氢酶的影响

Keywords: 马拉硫磷,双齿围沙蚕,乙酰胆碱酶,过氧化氢酶

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Abstract:

采用浓度梯度污染暴露室内模拟方法,研究了沙蚕暴露于不同浓度有机磷农药马拉硫磷的急性毒性效应,以及低浓度马拉硫磷对双齿围沙蚕乙酰胆碱酯酶(AChE)和过氧化氢酶(CAT)的影响。结果表明,马拉硫磷对沙蚕48、72、96h的LC50分别为71.68、49.21、33.16mg·L-1,安全浓度为3.32mg·L-1。在低浓度(0.0018~9mg·L-1)马拉硫磷中暴露3和6d,沙蚕体内乙酰胆碱酯酶活性受到显著抑制,暴露6d时9mg·L-1浓度组最高抑制率达88.92%,且乙酰胆碱酯酶抑制率与马拉硫磷浓度对数具有显著的剂量效应。沙蚕CAT活性随马拉硫磷浓度增加表现出先降后升再降的趋势,暴露3d时0.018mg·L-1浓度组CAT活性最高,6d时0.18mg·L-1浓度组CAT活性最高,分别比对照组高52.02%和53.42%(P<0.05),而浓度达1.8mg·L-1时CAT活力均显著下降(P<0.05)。结果显示,沙蚕乙酰胆碱酯酶是较好的滩涂有机磷农药污染生物标记物。

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