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铜胁迫和间作对玉米抗氧化酶活性及丙二醛含量的影响

DOI: 10.11654/jaes.2014.10.003, PP. 1890-1896

Keywords: ,抗氧化酶,丙二醛,间作

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Abstract:

为探讨红壤地玉米-豌豆间作种植模式对铜污染的响应机制,通过盆栽试验,研究了不同铜浓度(0、100、200、400、600mg·kg-1)对玉米单作和玉米间作豌豆条件下植株生物量、铜含量、玉米抗氧化酶活性超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)及丙二醛含量(MDA)的影响。结果表明:在高Cu2+浓度(600mg·kg-1)胁迫下,间作玉米地上部和地下部干重较单作分别提高了20%和36.6%,与单作相比,随着Cu2+浓度升高(100、200、400、600mg·kg-1),间作模式下玉米地上部铜含量分别降低了86.81%、44.57%、22.01%、11.11%,而地下部铜含量则分别提高了78.89%、24.79%、35.29%、13.31%,且差异均达到显着水平。在不同Cu2+浓度胁迫下,玉米叶和根中的SOD、POD、CAT活性和MDA含量均随着Cu2+浓度的增加而提高,仅Cu2+浓度达到600mg·kg-1时玉米体内的CAT活性有所下降。间作条件下玉米根的SOD活性较单作提高了48.07%~117.27%,间作玉米叶的SOD活性较单作提高了11.30%~46.90%.不同种植模式对Cu2+胁迫条件下的玉米POD活性均没有显着影响。在Cu2+(0~400mg·kg-1)胁迫下,间作玉米叶的CAT活性较单作均显着提高,分别提高了71.37%、140.40%、229.80%和161.75%,间作玉米根的CAT活性与单作无显着差异。间作玉米根的MDA含量较单作降低了26.13%~64.53%;在100、200mg·kg-1Cu2+胁迫下,间作玉米叶的MDA含量较单作降低了0.30%和26.24%,但在400、600mg·kg-1Cu2+胁迫下,间作玉米叶的MDA含量较单作提高了32.62%和93.51%.综上所述,在一定范围的Cu2+胁迫条件下,玉米根和叶中的抗氧化酶活性及MDA含量均有所提高来维持正常生长,间作模式在Cu2+胁迫下对玉米根和叶的抗氧化酶系统能起到一定的保护作用。

References

[1]  张文韬, 黄保健, 郭世荣, 等. 铜对空心菜光合作用及保护酶活性的影响[J]. 江苏农业学报, 2010, 26(2):303-307. ZHANG Wen-tao, HUANG Bao-jian, GUO Shi-rong, et al. Effects of copper on photosynthesis and protective enzyme activities of Ipomoea aquatica Forsk[J]. Jiangsu Agricultural Science, 2010, 26(2):303-307.
[2]  Bona E, Marsano F, Cavaletto M, et al. Proteomic characterization of copper stress response in Cannabis sativa roots[J]. Proteomics, 2007, 7(7):1121- 1130.
[3]  卫泽斌, 郭晓方, 丘锦荣, 等.间套作体系在污染土壤修复中的应用研究进展[J].农业环境科学学报, 2010, 29(增刊):267-272. WEI Ze-bin, GUO Xiao-fang, QIU Jin-rong, et al. Innovative technologies for soil remediation:Intercropping or co-cropping[J]. Journal of Agro-Environment Science, 2010, 29(Suppl):267-272.
[4]  蒋成爱, 吴启堂, 吴顺辉, 等.东南景天与不同植物混作对土壤重金属吸收的影响[J].中国环境科学, 2009, 29(9):985-990. JIANG Cheng-ai, WU Qi-tang, WU Shun-hui, et al. Effect of co-cropping Sedum alfredii with different plants on metal uptake[J]. China Environmental Science, 2009, 29(9):985-990.
[5]  王吉秀, 祖艳群, 李 元, 等. 玉米和不同蔬菜间套模式对重金属Pb、Cu、Cd累积的影响研究[J].农业环境科学学报, 2011, 30(11):2168-2173. WANG Ji-xiu, ZU Yan-qun, LI Yuan, et al. Effects of maize and vegetable intercropping system on accumulation of Pb, Cu and Cd in plants[J]. Journal of Agro- Environment Science, 2011, 30(11):2168-2173.
[6]  Xia H Y, Zhao J H, Sun J H, et al. Maize grain concentrations and above-ground shoot acquisition of micronutrients as affected by intercropping with turnip, faba bean, chickpea, and soybean[J]. Science China Life Sciences, 2013, 56(9):823-834.
[7]  An L Y, Pan Y H, Wang Z B, et al. Heavy metal absorption status of five plant species in monoculture and intercropping[J]. Plant and Soil, 2011, 345(1-2):237-245.
[8]  Jiang C A, Wu Q T, Sterckeman T, et al. Co-planting can phytoextract similar amounts of cadmium and zinc to mono-cropping from contaminated soils[J]. Ecological Engineering, 2010, 36:391-395.
[9]  Evans K, Gatehouse J, Lindsay W, et al. Expression of the pea metallothionein-like gene PsMTA in Escherichia coli and Arabidopsis thaliana and analysis of trace metal ion accumulation:Implications for PsMTA function[J]. Plant Molecular Biology, 1992, 20(6):1019-1028
[10]  袁玲花, 徐加宽, 颜士敏, 等. 土壤铜胁迫对不同籼型水稻品种产量和品质的影响[J]. 农业环境科学学报, 2008, 27(2):435-441. YUAN Ling-hua, XU Jia-kuan, YAN Shi-min, et al. Effects of soil Cu stress on grain yield and quality of indica rice cultivars[J]. Journal of Agro-Environment Science, 2008, 27(2):435-441.
[11]  江西省统计局. 江西统计年鉴[M]. 北京:中国统计出版社, 2013. Statistic Bureau of Jiangxi. Jiangxi statistical year book[M]. Beijing:China Statistice Press, 2013.
[12]  张志良, 瞿伟菁, 李小芳. 植物生理学实验指导[M]. 四版. 北京:高等教育出版社, 2009. ZHANG Zhi-liang, QU Wei-jing, LI Xiao-fang. Plant physiology experimental guidance[M]. Fourth edition. Beijing:Higher Education Press, 2009.
[13]  鲍士旦. 土壤农化分析[M]. 第三版. 北京:高等教育出版社, 2008. BAO Shi-dan. Soil Agricultural Chemistry Analysis[M]. Third edition, Beijing:Higher Education Press, 2008.
[14]  Rosenbaugh E G, Manickam D S, Batrakova E V, et al. Neuronal uptake and subcellular localization of functional nanoformulated copper/zinc superoxide dismutase(SOD nano)[J]. The FASEB Journal, 2012, 26:893. 2.
[15]  田胜尼, 彭少麟, 张玉琼, 等. 铜胁迫对鸭跖草的生长及生理特性的影响[J]. 中国农学通报, 2009, 25(9):144-147. TIAN Sheng-ni, PENG Shao-lin, ZHANG Yu-qiong, et al. The effects of copper stresses on the growth and physiological characteristics for Commelina communis[J]. Chinese Agricultural Science Bulletin, 2009, 25(9):144-147.
[16]  王松华, 张 华, 何庆元. 铜胁迫对紫花苜蓿幼苗叶片抗氧化系统的影响[J]. 应用生态学报, 2011, 22(9):2285-2290. WANG Song-hua, ZHANG Hua, HE Qing-yuan. Effects of copper stress on Medicago sativa seedlings leaf antioxidative system[J]. Chinese Journal of Applied Ecology, 2011, 22(9):2285-2290.
[17]  R Doctrow S, Liesa M, Melov S, et al. Salen Mn complexes are superoxide dismutase/catalase mimetics that protect the mitochondria[J]. Current Inorganic Chemistry, 2012, 2(3):325-334.
[18]  Jessica E S. Nanoceria exhibit redox state-dependent catalase mimetic activity[J]. Chemical Communications, 2010, 46(16):2736-2738.
[19]  Weismann D, Hartvigsen K, Lauer N, et al. Complement factor H binds malondialdehyde epitopes and protects from oxidative stress[J]. Nature, 2011, 478 (7367):76-81.
[20]  Dong Y J, Xu L L, Wang Q H, et al. Effects of exogenous nitric oxide on photosynthesis, antioxidative ability, and mineral element contents of perennial ryegrass under copper stress[J]. Journal of Plant Interactions, 2014, 9(1):402-411.
[21]  金 进, 叶亚新, 李 丹, 等. 重金属铜对玉米的影响[J]. 玉米科学, 2006, 14(3):83-86. JIN Jin, YE Ya-xin, LI Dan, et al. Effect of heavy metal(copper) coercion on maize[J]. Journal of Maize Sciences, 2006, 14(3):83-86.
[22]  Vural H, Demirin H, Kara Y, et al. Alterations of plasma magnesium, copper, zinc, iron and selenium concentrations and some related erythrocyte antioxidant enzyme activities in patients with Alzheimer\'s disease[J]. Journal of Trace Elements in Medicine and Biology, 2010, 24(3):169-173.
[23]  Thounaojam T C, Panda P, Mazumdar P, et al. Excess copper induced oxidative stress and response of antioxidants in rice[J]. Plant Physiology and Biochemistry, 2012, 53:33-39.
[24]  Drakiewicz M, Skrzyska-Polit E, Krupa Z. Copper-induced oxidative stress and antioxidant defence in Arabidopsis thaliana[J]. Biometals, 2004, 17(4 ):379-387.

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