全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

铅锌矿区重金属污染对微生物数量及放线菌群落结构的影响

Keywords: 铅锌矿区,微生物数量,放线菌,BOX-AIR,16S,rDNA,PCR-RFLP

Full-Text   Cite this paper   Add to My Lib

Abstract:

采集四川省汉源县富泉乡万顺铅锌矿区5个不同重金属浓度的土壤样品,进行了微生物数量及放线菌多样性的研究。经分离、纯化得到43株不同的放线菌,然后对其进行BOXAIR-PCR和16SrDNAPCR-RFLP分析。结果表明,铅锌矿区重金属复合污染对土壤微生物数量有较大的影响,随着铅锌矿区重金属污染程度的加剧,土壤微生物的总数下降。相关性分析表明,重金属含量与细菌数量呈极显著负相关(P<0.01),与放线菌数量、真菌数量呈显著负相关(P<0.05)。供试菌株的16SrDNA用HaeⅢ、HinfⅠ和TaqⅠ酶切后具有32种遗传图谱类型。BOXAIR-PCR的聚类结果表明在86%的水平上,所有菌株分为10个遗传类型,结果基本与16SrDNAPCR-RFLP聚类差异不大。来源于高重金属的含量样品的菌株基本聚在一起,可能是重金属含量影响了放线菌的分布。同时,16SrDNA序列聚类分析结合系统发育树分析表明链霉菌属是汉源铅锌矿区主要的放线菌属并且具有遗传多样性。

References

[1]  鲍士旦,土壤农化分析,北京:中国农业出版社,2000.
[2]  中国科学院南京土壤研究所,土壤理化分析,上海:上海科学技术出版社,1983.
[3]  Hu Qing;Qi Hong-yan;Zheng Jing-hai.Bacterial diversity in soils around a lead and zinc mine[J].Journal of Environmental Sciences,2007(1)
[4]  张汉波;于春蓓;施雯.不同时空铅锌矿渣中耐受重金属细菌群体的变化[J].农村生态环境,2004(01)
[5]  滕应;黄昌勇;龙健.铅锌银尾矿污染区土壤微生物区系及主要生理类群研究[J].农业环境科学学报,2003(04)
[6]  Caravaca F;Alguacil M M;Figueroa D,Re-establishment of Retama sphaerocarpa as a target species for reclamation of soil physical and biological properties in a semiarid Mediterranean land,Forest Ecology and Management? ,2003, 182.
[7]  Valery B;Eugene K,Soil surface geochemical anomaly around the copper-nickel metallurgical smelter,Water,Air & Soil Pollution,1998.
[8]  Kuperman RG ;Carreiro MM,Soil heavy metal concentrations, microbial biomass and enzyme activities in a contaminated grassland ecosystem.,Soil Biology and Biochemistry?,1997, 29(2).
[9]  Hiroki M,Decompesition of organic matter with previous cadmium adsorption in soils,Soil Science and Plant Nutrition,1994(02).
[10]  Ciller K E;Witter E;McGrath S P,Toxicity of heavy metals to microorganisms and microbial processes in agricultural soils:a review,Soil Biology&Biochemistry,1998.
[11]  Craig L M;Dobbs F C;Tiedje J M,Phylongenetic diversity of the bacterial community from microbial from microbial mat at an active,hydrothermal vent system.Loch Seamount,Hawaii,Applied and Environmental Microbiology,1995(61).
[12]  Kelly J J;H(a)ggblom M M;Tate R L,Effects of heavy metal contamination and remediation on soil microbial communities in the vicinity of a zinc smelter as indicated by analysis of microbial community phospholipid fatty acid profiles,Biology&Fertility of Soils,2003.
[13]  Mummey D L;Stahl P D;Buyer J S,Microbial biomarkers as an indicator of ecosystem recovery following surface mine reclamation,Applied Soil Ecology? ,2002, 21(03).
[14]  Waldrop M;Balser T;FirestoneM,Linking microbial community composition to function in a tropical soil,Soil Biology and Biochemistry,2000.
[15]  Amoroso MJ. ;Carlino FJ. ;Romero NC. ;Hill RT. ;Oliver G. ;Castro GR.,Screening of heavy metal-tolerant actinomycetes isolated from the Sali River,The Journal of General and Applied Microbiology?,1998, 44(2).
[16]  NIES D H,Microbial heavy--metal resistance,Applied Microbiology and Biotechnology? ,1999, 51.
[17]  Marta A P;Amoroso M J;Abate C M,Chromium(Ⅵ) resistance and removal by actinomycete strains isolated from sediments,Chemosphere,2007.
[18]  荆延德;何振立;杨肖娥.汞污染对水稻土微生物和酶活性的影响[J].应用生态学报,2009(01)
[19]  Melanie J Brown;Lester J N,Metal removal in activated sludge:the role of bacterial extra cellular polmers,Water Research? ,1980, 13.
[20]  Nriagu J O;Pacyna J M,Quantitative assessment of world-wide contamination of air,water and soils by trace metals,Nature? ,1988, 333.
[21]  Volesky B;Holan Z R,Biosorption of heavy-Metals,Biotechnology Progress? ,1995, 11.
[22]  Angela S;Birgit R;Ulrike P,Cultivation-independent population analysis of bacterial endophytes in three potato varieties based on eubacterial and Actinomycetes -specific PCR of 16S rRNA genes,FEMS Microbiology Ecology,2002(39).
[23]  刘杰;陈文新.我国中东部地区紫穗槐、紫荆、紫藤根瘤菌的数值分类及16S rDNA PCR-RFLP研究[J].中国农业科学,2003(01)
[24]  徐平;李文均;徐丽华.微波快速提取放线菌基因组DNA[J].微生物学通报,2003(04)
[25]  Versalovic J;Koeuty T;Lupski J R,Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes,Nucleic Acids Research,1991.
[26]  Lee Y K;Kim H W;Liu C L,A simple method for DNA extraction from marine bacteria that produce extracellular materials,Microbiological Methods,2003.
[27]  中国科学院南京土壤研究所微生物室,土壤微生物研究法,北京:科学出版社,1985.
[28]  鲁如坤,土壤农业化学分析方法,北京:中国农业科技出版社,2000.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133