全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

SO2诱导拟南芥气孔保卫细胞致死效应研究

Keywords: 拟南芥,SO2,细胞死亡,活性氧,Ca2+

Full-Text   Cite this paper   Add to My Lib

Abstract:

以拟南芥叶片下表皮为材料,研究了SO2体内衍生物——亚硫酸钠和亚硫酸氢钠混合液(3∶1,mmol·L-1/mmol·L-1)对气孔保卫细胞的致死作用。结果表明,浓度0.5~4.5mmol·L-1的SO2衍生物处理表皮3h可引起保卫细胞死亡,细胞死亡率呈浓度依赖性增高。抗坏血酸(AsA)或过氧化氢酶(CAT)与SO2衍生物共同作用时,保卫细胞死亡率显著降低。Ca2+螯合剂乙二醇双四乙酸(EGTA)或Ca2+通道抑制剂LaCl3与SO2衍生物共同作用时,保卫细胞死亡率亦显著降低。研究发现,一定浓度的SO2可诱导拟南芥保卫细胞死亡,胁迫可能通过诱导活性氧、激活质膜钙通道,造成胞外Ca2+内流,引发细胞死亡。

References

[1]  仪慧兰,姜林.SO2水合物诱发蚕豆(Viciafaba)根尖细胞染色体畸变效应[J].生态学报,2007,27(6):2318-2324.YI Hui-lan,JIANG Lin.Chromosomal aberrations induced by sulfur dioxide hydrates in Viciafaba root tips[J].Acta Ecologica Sinica,2007,27(6):2318-2324.
[2]  仪慧兰,李红孩,孟紫强.SO2体内衍生物诱发蚕豆根尖细胞微核[J].生态学报,2004,24(2):368-371.YI Hui-lan,LI Hong-hai,MENG Zi-qiang.Micronucleus induced by the derivatives of sulfur dioxide in Viciafaba root tips[J].Acta Ecologica Sinica,2004,24(2):368-371.
[3]  郝林,张惠文,徐昕,等.二氧化硫对小麦的氧化胁迫及其某些信号分子的调节[J].应用生态学报,2005,16(6):1038-1042.HAO Lin,ZHANG Hui-wen,XU Xi,et al.SO2-caused oxidative stress and modulation of some signal molecules in wheat[J].Chinese Journal of Applied Ecology,2005,16(6):1038-1042.
[4]  仪慧兰,李利红,仪民.二氧化硫胁迫导致拟南芥防护基因表达改变[J].生态学报,2009,29(4):1682-1687.YI Hui-lan,LI Li-hong,YI Min.Expression of Arabidopsis defenserelated genes in response to sulfur dioxide fumigation[J].Acta Ecologica Sinica,2009,29(4):1682-1687.
[5]  Jabs T.Reactive oxygen intermediates as mediators of programmed cell death in plants and animals[J].Biochemical Pharmacology,1999,57:231-245.
[6]  Mori I C,Schroeder J I.Reactive oxygen species activation of plant Ca2+ channels.A signaling mechanism in polar growth,hormone transduction,stress signaling,and hypothetically mechanotransduction[J].Plant Physiology,2004,135 (2):702-708.
[7]  lakimova E T,Wohering E J,Kapchina-Toteva V M,et al.Cadmium toxicity in cultured tomato cells-role of ethylene,pmteases and oxidative stress in cell death signaling[J].Cell Biology International,2008,32:1521-1529.
[8]  杜正清,孟紫强.二氧化硫衍生物和铅对大鼠神绛元膜离子通道损伤研究[D].太原:山西大学环境科学与上程研究中心,2005:1-106.DU Zheng-qing,MENG Zi-qiang.Effects of SO2 derivatives and lead on ion channel currents in acutely isolated rat neurons[D].Taiyuan:Research center of Environmental Science and Engineering,Shanxi University 2005:1-106.
[9]  曹更生,宋纯鹏.气孔保卫细胞信号转导途径[J].植物生理学通讯,1997,33(3):219-225.CAO C,eng-sheng,SONG Chun-peng.Signal transduction pathways in stomatal guard cells[J].Plant Physiology Communications,1997,33 (3):219-225.
[10]  Shapiro R.Genetic effects of bisulfite (sulfur dioxide)[J].Mutation Research,1977,38:149-176.
[11]  Larkin P J.Purification and viability determinations of plant protoplasts[J].Planta,1976,128:213-216.
[12]  Pfanz H,Martinoia E,Lange O L,et al.Flux of SO2 into leaf cells and cellular acidification by SO2[J].Plant Physiology,1987,85:928-933.
[13]  钱永常,余叔文.SO2对植物的氧化作用和植物的抗氧化作用[J].植物生理学通讯,1991,27(5):326-331.QIAN Yong-chang,YU Shu-wen.Oxidation of sulfur dioxide on plants and anti-oxidation of plants[J].Plant Physiology Communications,1991,27(5):326-331.
[14]  苗雨晨,宋纯鹏,董发才.ABA诱导蚕豆气孔保卫细胞H2O2的产生[J].植物生理学报,2000,26(1):53-58.MIAO Yu-chen,SUNG Chun-peng,DUNG Fa-cai.ABA-induced hydrogen peroxide generation in guard cells of Vicia faba[J].Acta Phytophysiologica Sinica,2000,26(1):53-58.
[15]  Smirnoff N.The function and metabolism of ascorbic acid in plants[J].Annals of Botany,1996,78:661-669.
[16]  Huang C H,He W L,Guo J K,et al.Increased sensitivity to salt stress in an ascorbate-deficient Arabidopsis mutant[J].Journal of Experimental Botany,2005,56(422):3041-3049.
[17]  程艳丽,宋纯鹏.植物细胞H2O2的信号转导途径[J].中国科学C辑生命科学,2005,35(6):480-489.CHENG Yan-li,SONG Chun-peng.H2O2 signal transduetion pathways in plant Cell[J].Science in china Set C,Life,Sciences,2005,35(6):480-489.
[18]  Errakhi R,Dauphin A,Meimoun P,et al.An early Ca2+ influx is a prerequisite to thaxtomin A-induced cell death in Arabidopsis thaliana cells[J].Journal of Experimental Botany,2008,59(15):4259-4270.
[19]  Lecourieux D,Mazars C,Pauly N,et al.Analysis and effects of cytosolic free calcium increases in response to elicitors in Nicotiana plum-baginifolia cells[J].Plant cell,2002,14(10):2627-2642.
[20]  Yakimova E T,Kapchina-Toteva V M,Woltering E J.Signal transduction events in aluminum-induced cell death in tomato suspension cells[J].Journal of Plant Physiology,2007,164:702-708.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133