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十溴联苯醚对土壤中微生物群落结构及土壤潜在硝化功能的影响

Keywords: 十溴联苯醚(BDE209),微生物群落结构,变性梯度凝胶电泳,荧光原位杂交

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Abstract:

采用变性梯度凝胶电泳(DGGE)和寡聚核苷酸探针-荧光原位杂交(FISH)方法研究了十溴联苯醚(BDE209)对土壤中微生物群落的影响作用。结果表明,1mg·kg-1BDE209对土壤中微生物群落多样性以及总细菌数有明显的促进作用,土壤中的氨氧化细菌以及亚硝酸氧化菌的数量有显著增长。BDE209浓度达到100mg·kg-1时,土壤微生物的总数和群落多样性则显著降低,同时氨氧化细菌以及亚硝酸氧化菌的生长也受到明显的抑制。暗室培养45d内,BDE209虽未被降解,但高浓度的十溴联苯醚会对土壤中的微生物群落结构及土壤潜在的硝化作用产生较大影响。

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