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一株Cr(Ⅵ)还原菌的筛选鉴定及其还原特性研究

DOI: 10.11654/jaes.2015.11.014

Keywords: 霍氏肠杆菌 Cr(Ⅵ) 还原菌 筛选鉴定

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Abstract:

从山西某铬渣堆场土壤中分离得到一株能还原Cr(Ⅵ)的细菌C2L,通过形态特征、生理生化反应和分子生物学鉴定确定该菌株为霍氏肠杆菌(Enterobacterhormaechei).该菌株能在较宽的pH 值(5~9)和温度(25~45 ℃)范围内还原Cr(Ⅵ),最佳反应条件为pH8和30 ℃.在Cr(Ⅵ)初始浓度为50、100、200、400、800 mg·L-1时,C2L菌株对Cr(Ⅵ)的还原效率分别为99.3%、94.2%、85.6%、82.1%、58.2%.实验结果表明,该菌株在高Cr(Ⅵ)浓度下仍可表现出良好的还原能力,具有独特的还原Cr(Ⅵ)性能,在处理Cr(Ⅵ)污染土壤中存在极大的应用潜力

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