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海洋科学  2011 

起始pH 值对海洋混合菌群产氢量的影响

, PP. 43-48

Keywords: 起始pH,产氢量,变性梯度凝胶电泳,16S,rRNA,基因,Fe-氢酶基因

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Abstract:

对采自天津潮间带的污泥进行热休克处理,厌氧条件下富集混合菌群进行产氢试验;设置起始pH从4.0到8.0(间隔0.5个单位),比较混合菌群在不同pH培养条件下产氢量;利用变性梯度凝胶电泳(DGGE)分析不同起始pH培养条件下的混合菌群组成。结果表明,在起始pH为7.0时,混合菌群产氢量最高。不同起始pH对产氢量的影响,依次为pH6.5>pH7.5>pH8.0>pH6.0>pH5.5>pH5.0>pH4.5>pH4.0。混合菌16SrRNA基因的PCR扩增产物经DGGE分离,电泳图谱表明,不同起始pH条件下混合菌群的优势菌相同,PCR产物的测序结果与Clostridiumsp.存在比较高相似度(98%)。Fe-氢酶基因扩增产物在DGGE电泳图谱中存在一条特异条带,其测定序列与Clostridiumperfringens氢酶基因相似度为98%。不同起始pH条件下混合菌群的产氢菌基本相同,混合菌群产氢量之间的差异可能在于起始pH对混合菌群Fe-氢酶基因活性的影响,有待进一步研究确定。

References

[1]  Valdez-Vazquez I;Ponce-Noyola M T;Poggi-Varaldo H M,Nutrients related to spore germination improve H:production from heat-shock-treated consortia,International Journal of Hydrogen Energy,2009(10).
[2]  Kataoka N;Miya K;Kiriyama K,Studies on hydrogen production by continuous culture system of hydrogen-producing anaerobic bacteria,Water Science and Technology,1997.
[3]  Kumar N;Das D,Enhancement of hydrogen production by Enterobaeter cloacae IIT-BT 08,Process Biochemistry,2000.
[4]  Lin, Chiu-Yue ;Chang, Rong-Chung,Hydrogen production during the anaerobic acidogenic conversion of glucose,Journal of Chemical Technology & Biotechnology?,1999, 74(6).
[5]  Valdez-Vazquez, I ;Rios-Leal, E ;Munoz-Paez, KM ;Carmona-Martinez, A ;Poggi-Varaldo, HM,Effect of inhibition treatment, type of inocula, and incubation temperature on batch H-2 production from organic solid waste,Biotechnology and Bioengineering?,2006, 95(3).
[6]  Levin DB;Pitt L;Love M,Bio-hydrogen production:prospects and limitations to practical application,International Journal of Hydrogen Energy,2004.
[7]  Mizno O;Dinsdale R;Hawkes F R,Enhancement of hydrogen production from nitrogen gas sparging,Bioresource Technology,2000.
[8]  Mizno O;Ohara T;Shinya M,Characteristics of hydrogen production from bean curd manufacturing waste by anaerobic microflora,Water Science and Technology,2000.
[9]  Sparling R;Risbey D;Poggi-Varaldo H M,Hydrogen production from inhibited anaerobic composters,International Journal of Hydrogen Energy,1997.
[10]  Noike T;Mizno O,Hydrogen fermentation of organic municipal wastes,Water Science and Technology,2000.
[11]  Okamoto M;Miyahara T;Mizno O,Biological hydrogen potential of materials characteristic of theorganic fraction of municipal solid wastes,Water Science and Technology,2000.
[12]  Fang HHP. ;Liu H.,Effect of pH on hydrogen production from glucose by a mixed culture,Bioresource Technology?,2002, 82(1).
[13]  Jun, Yoon-Sun ;Seung-Ho Yu ;Keun-Garp Ryu ;Tae-Jin Lee,Kinetic Study of pH Effects on Biological Hydrogen Production by a Mixed Culture,Journal of microbiology and biotechnology?,2008, 18(6).
[14]  Nicolaisen MH ;Ramsing NB,Denaturing gradient gel electrophoresis (DGGE) approaches to study the diversity of ammonia-oxidizing bacteria,Journal of Microbiological Methods?,2002, 50(2).
[15]  Fang H H P;Zhang T;Li C L,Characterization of Fe-hydrogenase genes diversity and hydrogen-producing population in an acidophilic sludge,Journal of Biotechnology,2006.
[16]  Chang J J;Chen W E;Shih S Y,Molecular detection of the clostridia in an anaerobic biohydrogen fermentation system by hydrogenase mRNA-targeted reverse transcription-PCR,Applied Microbiology and Biotechnology,2006.
[17]  Hiroshi Y;Miyoko W;Naoko M,Effect of fermentation temperature on hydrogen production from cow waste slurry by using anaerobic microflora within the slurry,Applied Microbiology and Biotechnology,2007.
[18]  Dealing Zhu ;Guangce Wang ;Hongjin Qiao ;Jinling Cai,Fermentative Hydrogen Production By The New Marine Pantoea Agglomerans Isolated From The Mangrove Sludge,International Journal of Hydrogen Energy?,2008, 33(21).
[19]  邢德峰;任南琪;宫曼丽.DGGE技术监测生物制氢反应器微生物群落结构和演替[J].中国科学C辑,2004(6)
[20]  Ren N;Wang B;Ma F,Hydrogen bio-production of carbohydrate fermentation by anaerobic sludge process,Miami,1995.
[21]  Lee Y J;Miyahara T;Noike T,Effect of pH on the microbial hydrogen fermentation,Journal of Chemical Technology and Biotechnology,2002(6).
[22]  Lay J J Modeling and optimization of anaerobic digested sludge converting starch to hydrogen [J] 2000(3)
[23]  Dabrock B;Bahl H;Gottschalk G,Parameters affection solvent production by Clostridium pasteuriamum,Applied and Environmental Microbiology,1992(4).
[24]  Li C L;Fang H H P,Fermentative hydrogen production from wastewater and solid wastes by mixed cultures,Critical Reviews in Environmental Science and Technology,2007.
[25]  Wang J L;Wan W,Comparison of different pretreatment methods for enriching hydrogen-producing cul-tures from digested sludge,International Journal of Hydrogen Energy,2008.
[26]  Ayhan Demirbas,Progress and recent trends in biofuels,Progress in Energy and Combustion Science?,2007, 33(1).

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