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海洋科学  2011 

矛尾复虾虎鱼溃疡病病原创伤弧菌的鉴定

, PP. 20-25

Keywords: 矛尾复虾虎鱼(Synechogobius,hasta),创伤弧菌(Vibrio,vulnificus),16S,rRNA,基因,gyrB基因

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Abstract:

2009年8月江苏连云港赣榆县多家虾蟹养殖塘混养的矛尾复虾虎鱼(Synechogobiushasta)大量死亡,从病鱼深层溃烂组织及肝脏中分离出大量优势生长的细菌,人工感染试验证明分离菌对虾虎鱼的半数致死量(LD50)为1.63×106cfu/g。对分离菌进行了形态特征、理化特性和分子生物学等方面的分析。一方面,分离菌株16SrRNA和gyrB基因序列的BLAST搜索及最大简约法分析均表明其与创伤弧菌(Vibriovulnificus)亲缘关系最近;另一方面,分离菌革兰氏阴性,氧化酶阳性,葡萄糖发酵型。因此,引起江苏连云港赣榆县矛尾复虾虎鱼溃疡病病原菌是弧菌属的创伤弧菌。

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