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海洋科学  2012 

海洋红树林植物黄槿内生真菌Aspergillus sydowii EN-198化学成分研究

, PP. 6-11

Keywords: 红树林,黄槿,内生真菌,化学成分,结构鉴定

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Abstract:

对采自海南东寨港的海洋红树林植物黄瑾(Hibiscustiliaceus)叶中分离到的一株内生真菌AspergillussydowiiEN-198的次生代谢产物进行了研究。利用正相与反相硅胶柱层析、葡聚糖凝胶SephadexLH-20柱层析以及制备薄层层析(PTLC)等分离手段从其发酵液中分离得到14个化合物,通过一维、二维核磁共振技术、质谱技术等鉴定了所有化合物的结构,分别为:环-(S-脯氨酸-S-苯丙氨酸)(1),环-(S-脯氨酸-S-亮氨酸)(2),环-(S-苯丙氨酸-S-色氨酸)(3),(1S)-1-(4′-间羟基苯甲酸)-1,1,5,5-二甲基己二醇(4),曲酸(5),N-2-(4-吲哚)乙基乙酰胺(6),N-2-(4-对羟基苯酚)乙基乙酰胺(7),胸腺嘧啶脱氧核苷(8),尿嘧啶脱氧核苷(9),尿嘧啶核苷(10),过氧化麦角甾醇(11),麦角甾醇(12),(2S,2′R,3R,3′E,4E,8E)-N-(2′-羟基-3′-十六烯酰基)-9-甲基-4,8-二十碳二烯-1,3-二醇(13)以及1-O-β-D-葡萄糖基-(2S,2′R,3R,3′E,4E,8E)-N-(2′-羟基-3′-十六烯酰基)-9-甲基-4,8-二十碳二烯-1,3-二醇(14);其中化合物1和2为首次从Aspergillussydowii中分离得到。对所有化合物测试其抑菌活性,发现化合物5与7对金黄色葡萄球菌有较好抑制活性。

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