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海洋科学  2012 

牙鲆RAG2 基因的质粒构建和体外原核表达

, PP. 59-63

Keywords: 牙鲆(Paralichthys,olivaceus),RAG2,重组,纯化

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Abstract:

将牙鲆(Paralichthysolivaceus)RAG2cDNA序列插入到体外表达质粒pProEXTMHT,在大肠杆菌(Escherichiacoli)BL-21中进行体外表达,分析了诱导时间和IPTG浓度对重组蛋白产生量的影响,确定了最佳诱导条件为:诱导时间为3h,IPTG诱导浓度0.2mmol/L。本研究同时对RAG2重组蛋白的可溶性进行确认,并利用BDTALONTM金属亲和柱纯化了RAG2蛋白。本研究结果为进一步深入研究RAG2蛋白的生物学功能奠定了良好的基础。

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