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海洋科学  2012 

基于LC-MS/MS 技术对蓝藻Gloeobacter violaceus PCC 7421细胞膜的蛋白质组学研究

, PP. 39-44

Keywords: Gloeobacter,violaceus,PCC,7421,LC-MS/MS,细胞质膜

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Abstract:

GloeobacterviolaceusPCC7421是一种在进化上很古老的无类囊体蓝藻,由于光合作用电子传递链与呼吸系统在其细胞质膜上共存,这两个系统可能会共享一些元件,为研究其光合作用系统结构的独特之处,应用高效液相色谱-串联质谱联用(LC-MS/MS)技术对其细胞膜蛋白进行蛋白质组学分析。经鉴定发现,G.violaceus的细胞质膜上具有PSⅠ、PSⅡ和细胞色素b6等光合作用关键蛋白,同时具有呼吸作用过程中的关键酶1,5-二磷酸羧化酶/加氧酶、F0F1-ATP酶α和β亚基、细胞分裂蛋白、硝酸盐/亚硝酸盐转运蛋白、青霉素结合蛋白以及多种药物受体ABC转运蛋白。由此可知,G.violaceus的细胞质膜不仅能够同时进行光合作用和呼吸作用,且营养盐运输等转运功能也同时存在。G.violaceus的全基因数据分析显示其基因序列具有独特性,其蛋白注释相对比较少,因此其蛋白功能的研究需要更多数据的支持。

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