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海洋科学 2012

海湾扇贝与紫扇贝生殖隔离研究

, PP. 9-14

张守都,张国范,李莉

Keywords: 海湾扇贝(Argopecten,irradians),秘鲁紫扇贝(Argopecten,purpuratus),种间杂交,ITS1,合子后隔离

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Abstract:

对海湾扇贝(Argopectenirradians)和秘鲁紫扇贝(Argopectenpurpuratus)进行了跨种间的杂交尝试,结果显示两个物种之间正向和反向均可受精和正常发育,没有发现合子前隔离,并且通过internaltranscribedspacer1(ITS1)标记验证了杂交的成功。至杂交子一代性成熟时通过对杂合子成熟性腺进行H.E.染色石蜡切片观察发现,雄性性腺明显退化且不能产生有活力的精子,雌性性腺发达且能形成外观正常卵子,但是却不能与亲本进行回交,即表现为典型的合子后隔离:杂种不育。

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