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海洋科学  2006 

磷沙蚕凝集素的分离纯化及生物活性研究

, PP. 59-64

Keywords: 磷沙蚕(Chaetopterus,variopedatus),凝集素,β-葡萄糖苷酶,NB4白血病细胞株,脾细胞

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Abstract:

采用DEAE-Toyopearl阴离子交换树脂、Bio-GelP-100分子筛层析、Lactosyl-Sepharose4B亲和层析和Sephadex75分子筛层析技术从日本海的海洋无脊椎动物磷沙蚕(Chaetopterusvariopedatus)中分离出2种凝集素,通过体外细胞活性和酶活性实验观察它们的生物活性。结果表明,从海洋无脊椎动物磷沙蚕中分离出一种β-半乳糖特异性凝集素(CVL)和一种具有血凝活性,但不被经测试的单糖和糖蛋白所抑制的相对分子质量为30000的蛋白质(CVL-1)。β-半乳糖特异性凝集素(CVL)具有体外抑制NB4白血病细胞株的活性和抑制小鼠脾细胞的活性,IC50分别为7.87mg/L和22mg/L。同时,另一种蛋白质(CVL-1)具有β-葡萄糖苷酶活性。

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