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海洋科学  2006 

基于SAMPL的一种微卫星筛选方法在中国明对虾中的尝试

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Keywords: 选择性扩增多态微卫星位点(SAMPL),中国明对虾(Fenneropenaeus,chinensis),微卫星

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Abstract:

微卫星的发展主要受限于微卫星及其旁侧特异引物区的分离,在中国明对虾(Fenneropenaeuschinensis)中建立了选择性扩增多态微卫星位点(SAMPL,selectiveamplifiedmic-rosatellitepolymorphicloci)技术体系,并初步尝试了基于SAMPL的一种微卫星筛选方法。通过5′端锚定引物引入微卫星序列,对传统SAMPL技术加以改进并以期富集简单重复序列。从测序胶上随机选择5条SAMPL条带(分别命名为S11,S13,S14,S22,S24)克隆测序,大小分别为161,157,147,152,298。其中S11,S13,S14,S22四个片段两端引物分别为AFLP选择性引物和SAMPL引物,S13和S22含微卫星序列重复,S24两端引物均为传统的AFLP引物,在此片断中有一(AG)39重复。

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