全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
海洋科学  2005 

单条固定线虫基因组DNA提取及18S rRNA基因PCR扩增

, PP. 33-36

Keywords: 线虫,固定,基因组DNA,18S核糖体RNA基因

Full-Text   Cite this paper   Add to My Lib

Abstract:

根据线虫18S核糖体RNA基因PCR扩增效果比较了丙酮、乙醇、乙醇+0.05mol/LEDTA(pH8.0)和5%海水福尔马林4种固定剂,碱裂解和蛋白酶K处理2种单条线虫基因组DNA提取方法的优劣。用乙醇固定的样品最适合制备PCR模板DNA,而5%海水福尔马林固定的样品能最完整地保持样品形态。蛋白酶K处理获得的DNA较碱裂解获得的更适合PCR扩增。结果有助于分子生物学方法在海洋线虫分类、多样性和生态学研究中的应用。

References

[1]  杨官品 朱艳红.土壤细菌基因资源的直接分离——16S核糖体RNA基因模式[J].湖北大学学报:自然科学版,:.
[2]  张志南 周红 等.胶州湾小型底栖生物的丰度和生物量[J].海洋与湖沼,:.
[3]  张志南 周红 等.渤海线虫群落的多样性及中性模型分析[J].生态学报,:.
[4]  徐来祥 张知彬 等.福尔马林保存的动物标本基因组DNA的提取方法[J].动物学报,:.
[5]  赵春江 李宁.一种从毛发中提取DNA的简易方法[J].遗传,:.
[6]  Parmesan C, Yohe G. Aglobally coherent fingerprint of climate change impacts across nature systems[J]. Nature, 2003, 421:37- 42.
[7]  Root T L, Price J T, Hall K R, et al. Fingerprints of global warming on wild animals and plants[J]. Nature, 2003, 421:57 - 60.
[8]  Zingone A, Oksfeldt - Eneroldsen H. The diversity of harmful blooms: a challenge for science and management[J]. Ocean and Coastal Management, 2000, 43: 725- 748.
[9]  The C. elegans Sequencing Consortium. G enome sequence of the nematode C. elegans: A platform for investigating biology[J]. Nature, 1998, 282:2 012- 2 018.
[10]  Kamath R S, Fraser A G, Dong Y, et al. Systematic functional analysis of the Caenorthabditis elegans genome using RNAi [J ].Nature, 2003, 421: 231-237.
[11]  Blaxter M L, De Ley P, Garey J R. Amolecular evolutionary framework for the phylum nematoda[J] . Nature, 1998,392:71 - 75.
[12]  Floyd R M, Abebe E, Papert A. Molecular barcodes for soil nematode identification[J ]. Molecular Ecology, 2002, 11:839 - 850.
[13]  Dorris M, De Ley P, Blaxter M L. Molecular analysis od nematode diversity and the evolution of oarasitism[J]. Parasitology Today, 1999, 15: 188-193.
[14]  Platt H M, Warwick R M. Free - living Marine Nematodes[M]. Cambridge, London: Cambridge University Press, 1983.
[15]  Lambshead P J D, TietjenT, GloverA. Impact of large scale natural physical disturbance of the diversity of deep - sea North Atlantic nematodes[J]. Marine Ecology, 2001, 214:121-126.
[16]  KimJ, Chae C. Optimized protocols for the detection of porcine circovirus2 DNA from formalin - fixed paraffin - embedded tissues using nested polymerase chain reaction and comparison of nested PCR with in situ hybridization [J] . Journal of Virological Methods, 2001, 92: 105-111.
[17]  Fukatsu T. Acetone preservation: a practical technique for molecular analysis [J]. Molecular Ecology, 1999, 8:1935 - 1945.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133