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海洋科学  2006 

南极产低温几丁质酶菌株的筛选及分子鉴定

, PP. 35-38

Keywords: 南极,低温几丁质酶,嗜冷菌,分子鉴定

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Abstract:

从南极普里兹湾深海底泥样品中分离得到一批嗜冷细菌,对这些嗜冷细菌进行了产几丁质酶菌株的筛选,对其中一株具有较高产酶活性的菌株AC167所分泌的几丁质酶进行了性质分析,并通过16SrDNA序列分析进行了菌株的分子鉴定。该菌株最适生长温度为10℃,最高生长温度为25℃,是典型的嗜冷菌,经16SrDNA序列比较及系统发育分析鉴定为假单胞菌属(Pseudomonas)。该菌株只在低于25℃的条件下分泌几丁质酶,酶的最适反应温度为30°C,属于低温酶。

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