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海洋科学  2008 

用显微注射方法分析牙鲆肌肉发育调节基因MyoD, Myf5的启动子序列

, PP. 31-35

Keywords: 肌肉发育,启动子,牙鲆(Paralichthys,olivaceus)

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Abstract:

克隆了牙鲆(Paralichthysolivaceus)肌肉发育调节基因MyoD和Myf5的启动子序列,分别为608bp和1073bp。对序列进行分析发现MyoD和Myf5的启动子含有参与肌肉发育调控的基因的结合位点。将MyoD和Myf5的启动子连接到GFP载体上,构建了重组质粒MyoDP-GFP和Myf5P-GFP,并注射到一细胞或两细胞的斑马鱼胚胎中,对这两个基因的启动子进行了瞬时表达研究。结果表明,牙鲆MyoD和Myf5的启动子可以驱动绿色荧光蛋白特异地在斑马鱼肌肉纤维中表达,因此牙鲆608bpMyoD和1073bpMyf5的启动子包含着可以驱动MyoD和Myf5正常表达的必需元件,它们是肌肉特异的,并且可以跨物种行使其功能。

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