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海洋科学  2010 

除草剂草丁膦抗性基因bar作为三角褐指藻基因工程选择标记的研究

, PP. 62-66

Keywords: 三角褐指藻(Phaeodactylum,tricornutum),草丁膦,bar基因,基因工程,选择标记

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Abstract:

研究了三角褐指藻(Phaeodactylumtricornutum)对氯霉素、卡那霉素、青霉素、链霉素、潮霉素5种抗生素和一种除草剂草丁膦的敏感性,发现三角褐指藻对草丁膦非常敏感,用统计学分析法得出了其72h半致死质量浓度为22mg/L。通过基因枪法将携带草丁膦抗性基因bar的载体pSVB导入三角褐指藻细胞中,通过草丁膦筛选成功获得了抗性藻细胞,PCR检测获得阳性结果,提示草丁膦更适合用于构建安全、高效、廉价的三角褐指藻表达系统。

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