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海洋科学  2011 

条斑紫菜低覆盖度基因组草图分析

, PP. 76-81

Keywords: 条斑紫菜(Porphyra,yezoensis),Solexa,高通量测序,代谢通路

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Abstract:

对条斑紫菜(Porphyrayezoensis)进行Solexa高通量测序,获得低覆盖度全基因组草图。该基因组草图大小约220Mbp,GC质量分数53.08%;包含26629个预测基因,其中16409个基因具有内含子,平均每个基因含2.22个内含子;基因结构分析表明具有内含子的基因平均长度为2214bp,内含子平均大小319bp;代谢通路分析表明嘌呤代谢是含有蛋白数量最多的代谢途径。本研究所得条斑紫菜低覆盖度全基因组草图快速获取了基因组大小和蛋白编码基因结构等基本信息,证明了使用Solexa高通量测序技术对条斑紫菜进行全基因组测序的可行性。

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