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海洋科学  2006 

夏牙鲆(♂)与牙鲆(♀)人工杂交的细胞遗传学初步研究

, PP. 51-55

Keywords: 夏牙鲆(Paralichthys,dentatus),牙鲆(Paralichthys,olivaceus),人工杂交,细胞遗传

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Abstract:

于2004年11~12月在青岛薛家岛养鱼场采用夏牙鲆(Paralichthysdentatus)的精子与牙鲆(Paralichthysolivaceus)的卵子实施了多次人工杂交实验,并进行了父本母本鱼类个体、子代的染色体制片观察和细胞中DNA相对含量的流式细胞仪检测。结果表明,夏牙鲆染色体数为48,核型为2n=48t、臂数NF=48;子代的染色体数和核型与父母本的一样,也为2n=48t;子代的细胞中DNA的相对含量也表明其为二倍体。因此,可以明确其为杂交的后代,而不是雌核发育。

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