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海洋科学  2007 

雨生红球藻β-胡萝卜素酮化酶基因在莱茵衣藻叶绿体中的表达

, PP. 67-72

Keywords: 莱茵衣藻(Chlamydomonas,reinhardtii),叶绿体转化,β-胡萝卜素酮化酶基因(bkt)

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Abstract:

雨生红球藻(Haematococcuspluvialis)的β-胡萝卜素酮化酶是虾青素生物合成途径中关键酶之一,本研究通过基因枪法将克隆出的β-胡萝卜素酮化酶基因(bkt),转入莱茵衣藻(Chlamydomonasreinhardtii)的叶绿体中,经过含100mg/L壮观霉素固体培养基筛选得到转化子。通过PCR和基因组酶切Southern杂交分析,证明bkt基因通过同源重组定点整合到转化子的叶绿体基因组中。进一步通过RT-PCR和RT-PCRSouthern杂交分析,表明bkt在衣藻转化子中得到表达。

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