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海洋科学  2007 

大菱鲆两种转基因方法的比较研究

, PP. 9-13

Keywords: 转基因效率,电脉冲精子介导法,转染,大菱鲆(Scophthat,musmaximus)

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Abstract:

对外源基因导入大菱鲆(Scophthatmusmaximus)的两种方法进行了研究,采用经过改进的电脉冲方法以及哺乳动物细胞中常用的转染法导入外源基因,并对两种方法进行比较。电脉冲最佳导入条件为:脉冲电压为300V/cm,脉冲次数为5,外源DNA质量浓度为20mg/L;转染法的最佳操作时间为受精后50min。应用上述两种方法进行批量转基因鱼实验,得到外源基因的导入率分别为20%~28%和60%~70%。实验表明利用转染试剂jetPEI处理单细胞期的大菱鲆受精卵能得到较高的孵化率及较高的转基因效率。

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